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Streptococcus thermophilus capable of producing gamma-aminobutyric acid at high yield and efficiently converting sodium glutamate as well as culture method and application of streptococcus thermophilus

A technology of Streptococcus thermophilus and GABA, applied in the direction of microorganism-based methods, Streptococcus/Lactococcus, biochemical equipment and methods, etc., can solve the problem of low production of GABA and improve human health , increase production, and ensure the effect of fermentation

Pending Publication Date: 2022-05-06
ZHEJIANG INM FOOD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in related technologies, the yields of microbial fermentation of gamma-aminobutyric acid are low, so the inventors conducted research on how to increase the yield of microbial fermentation of gamma-aminobutyric acid

Method used

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  • Streptococcus thermophilus capable of producing gamma-aminobutyric acid at high yield and efficiently converting sodium glutamate as well as culture method and application of streptococcus thermophilus
  • Streptococcus thermophilus capable of producing gamma-aminobutyric acid at high yield and efficiently converting sodium glutamate as well as culture method and application of streptococcus thermophilus

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0046] Prepare seed liquid, comprise the following steps:

[0047] S1. Isolation of the strain: A Streptococcus thermophilus with high yield of γ-aminobutyric acid and efficient conversion of glutamic acid was isolated and screened from traditional fermented yak yogurt made by herdsmen in Ruoergai County, Aba Prefecture, Sichuan Province. The preservation number of this Streptococcus thermophilus is CGMCC No.23415, classified as Streptococcus thermophilus. The strain is currently preserved in the General Microbiology Center of the China Committee for the Preservation of Microbial Strains. The address of the preservation unit is: Beijing, China, and the preservation date is September 15, 2021.

[0048] S2, Activation of strains:

[0049] Weigh 76.1g of MC medium and dissolve it in 1L of water, autoclave at 121°C for 15 minutes, cool to 50°C, and pour it into a sterile petri dish to prepare MC plates;

[0050]Using the inoculation loop repeatedly burned on the alcohol lamp, ta...

Embodiment 1

[0060] A method for preparing fermented skim milk, comprising the following steps:

[0061] S1, add the seed liquid in Preparation Example 1 to the fermentation substrate, the volume ratio of the seed liquid and the fermentation substrate is 1:50, and the fermentation substrate is sterilized at 105°C for 15 minutes;

[0062] S2, ferment in a constant temperature incubator at 37°C for 48 hours to obtain fermented skim milk.

[0063] In terms of mass percentage, the fermentation substrate includes: 10% skimmed milk powder, 1.0% (wt / vol) sodium glutamate, and the balance is water.

[0064] In other embodiments, white sugar and other common raw materials in fermented milk in the prior art can also be added to the fermentation substrate.

Embodiment 2

[0066] A method for preparing fermented skim milk, the difference from Example 1 is that the fermentation substrate does not contain sodium glutamate.

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Abstract

The invention relates to the field of microorganisms, and particularly discloses streptococcus thermophilus capable of producing gamma-aminobutyric acid at high yield and efficiently converting sodium glutamate as well as a culture method and application of the streptococcus thermophilus. The invention discloses streptococcus thermophilus capable of producing gamma-aminobutyric acid at high yield and efficiently converting sodium glutamate. The preservation number of the streptococcus thermophilus is CGMCC (China General Microbiological Culture Collection Center) No.23415. The strain can be fermented to generate a large amount of gamma-aminobutyric acid, the yield of gamma-aminobutyric acid in a fermented product can reach 1.54-2.62 mg / mL, on one hand, the yield of gamma-aminobutyric acid is effectively increased, and on the other hand, the cost of adding gamma-aminobutyric acid is effectively reduced. Meanwhile, the streptococcus thermophilus has the capacity of efficiently converting sodium glutamate, the conversion efficiency of the streptococcus thermophilus to the sodium glutamate is close to 100%, and the defect that the residual quantity of the sodium glutamate is high in the related technology can be effectively overcome.

Description

technical field [0001] The application relates to the field of microbes, more specifically, it relates to a strain of Streptococcus thermophilus capable of high-yielding gamma-aminobutyric acid and efficiently transforming sodium glutamate, and its culture method and application. Background technique [0002] γ-aminobutyric acid (GABA for short) is a naturally occurring non-protein amino acid, widely distributed and present in animals and plants, and also an important inhibitory neurotransmitter in the mammalian central nervous system, about 30 % of the central nervous system uses γ-aminobutyric acid as a transmitter to promote blood circulation and oxygen supply in the brain, and plays an important role in the human cerebral cortex, hippocampus, thalamus, basal ganglia and cerebellum Its multiple functions have regulatory effects, such as relieving the tension of the brain, promoting good sleep and the synthesis of growth hormone. [0003] With the growth of age and the ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00A23C9/123C12R1/46
CPCC12N1/20C12P13/005A23C9/1238A23V2400/249Y02P60/87
Inventor 鄢明辉王伟军杨欢东洪环陈波朱立科
Owner ZHEJIANG INM FOOD