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A method for inhibiting browning of chitosan oligosaccharides

A technology of chitosan oligosaccharide and browning, which is applied in the fields of enzyme engineering and fermentation engineering, can solve problems such as limiting the popularization and application of chitosan oligosaccharide, reducing biological activity, and affecting product quality, so as to reduce amino browning reaction, convenient operation, and low cost. low effect

Active Publication Date: 2022-06-28
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Browning of chitosan oligosaccharides will reduce its purity, affect product quality, and also reduce its biological activity
At present, the oligochitosaccharides sold on the market generally have problems such as high browning degree and too dark product color, which limit the popularization and application of oligochitosaccharides and related products.

Method used

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  • A method for inhibiting browning of chitosan oligosaccharides
  • A method for inhibiting browning of chitosan oligosaccharides
  • A method for inhibiting browning of chitosan oligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Influence of metal ions on browning of chitosan hydrolyzed solution To 600 mL of chitosan acetic acid solution with a concentration of 0.10 g / mL, 6 mL of chitosanase-containing solution (the amount of enzyme added is 2.5 U / mL) was added. g chitosan). Divide into 6 parts, take 5 parts of them, add MgCl respectively 2 , MnCl 2 , CaCl 2 , KCl, BaCl 2 , the final concentration of metal ions was 1 mmol / L, and the last part without metal ions was used as a control to react at 55 °C, 200 rpm water bath shaker for 6 hours; the enzymatic hydrolysis solution was diluted with water, and the volume of the enzymatic hydrolysis solution and water was The ratio was 1:9, the absorbance was measured at 420 nm, and the measured value was used as the browning index to explore the effect of metal ions on the browning of chitosan oligosaccharides.

[0029] The result is as figure 1 As shown, when there is 1 mmol / L Mn in the enzymatic hydrolysate, compared with no metal ions a...

Embodiment 2

[0030] Example 2 Browning control of chitosan hydrolyzed solution To 500 mL of chitosan acetic acid solution with a concentration of 0.10 g / mL, 5 mL of chitosanase-containing solution (the amount of added enzyme was 2.5 U / g of chitosan) was added. ). Divide into 5 parts, take 4 parts of them, add MnCl respectively 2, so that the final concentrations were 1 mmol / L, 2 mmol / L, 5 mmol / L, 10 mmol / L, respectively, and the last part did not add MnCl 2 , as a control, the reaction was carried out at 55°C and 200 rpm in a water bath shaker, and samples were taken at intervals of 1 hour from the start of the reaction. Dilute the enzymatic hydrolysis solution in different time periods (1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours) with water. The absorbance value was measured at lower temperature, and the measured value was used as the browning index to explore the optimal Mn to inhibit the browning of chitosan oligosaccharide. 2+ concentration.

[0031] The experimen...

Embodiment 3

[0033] Example 3 Changes in the final product of enzymatic hydrolysis To 200 mL of 0.10 g / mL chitosan acetic acid solution, 2 mL of chitosanase-containing solution (the amount of enzyme added is 2.5 U / g of chitosan) is added. Divide into 2 parts, one of which is added with MnCl 2 , so that the final concentration is 5 mmol / L (as the experimental group), and the other part does not add Mn 2+ (as a control group), the reaction was carried out at 55° C., 200 rpm in a water bath shaker, and samples were taken at intervals of 1 hour from the start of the reaction.

[0034] Thin-layer chromatography (TLC) was used to analyze the product of the hydrolysis of chitosan by the enzyme solution, and the obtained sample was spotted and then developed twice in a developing solvent (isopropanol-water-ammonia with a volume ratio of 8:3:1). , after the development is finished, blow dry with a hair dryer and dye with a dye (0.1% ninhydrin-ethanol solution), develop the color at 110 °C for 10 m...

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Abstract

The invention discloses a method for inhibiting the browning of chitosan oligosaccharides, which belongs to the fields of fermentation engineering and enzyme engineering. The specific method of the invention is: adding Mn to chitosan oligosaccharide solution or the reaction solution for preparing chitosan oligosaccharides 2+ Make its final concentration 1-10 mmol / L. The Mn 2+ is added in the form of a manganese salt which may be selected from MnCl 2 . The invention also discloses Mn 2+ Application in inhibiting browning of chitosan oligosaccharides. The present invention adds Mn in the reaction solution that prepares chitosan oligosaccharide 2+ , through experimental research, it can effectively inhibit the browning of chitosan oligosaccharides. The method of the invention can directly inhibit the browning of the chitosan oligosaccharide, reduce the browning degree of the chitosan oligosaccharide, and has the advantages of wide application, low cost and convenient operation.

Description

technical field [0001] The invention relates to a method for inhibiting browning of chitosan oligosaccharide, belonging to the fields of fermentation engineering and enzyme engineering. Background technique [0002] Chitosan oligosaccharide is a bioactive small-molecule oligosaccharide derived from the ocean. It is obtained from the degradation of macromolecular chitosan, and its molecular weight is less than 3.2 kDa. Chitosan oligosaccharide, as a hydrolyzate of chitosan, has the characteristics of low molecular weight, good water solubility, high cell permeability, unique biological activity, and easy absorption and utilization by the body. It has been widely researched and applied in the fields of agriculture and daily chemical industry, and it is a marine functional oligosaccharide with broad application prospects. [0003] At present, the preparation methods of chitosan oligosaccharide mainly include physical method, chemical method and enzymatic hydrolysis method. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00C12P19/14
CPCC12P19/00C12P19/14
Inventor 毛相朝孙建安王永臻苏海鹏贾真荣
Owner OCEAN UNIV OF CHINA
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