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Neuron antigen spectrum antibody detection kit and detection method thereof

A technology of antibody detection and neurons, applied in the field of kits, to achieve the effects of high sensitivity, simple operation, convenient and rapid in vitro disease diagnosis

Pending Publication Date: 2022-05-06
苏州邦器生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Positive results for antibodies to the above antigens may precede the diagnosis of the tumor

Method used

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  • Neuron antigen spectrum antibody detection kit and detection method thereof
  • Neuron antigen spectrum antibody detection kit and detection method thereof
  • Neuron antigen spectrum antibody detection kit and detection method thereof

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Experimental program
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Embodiment Construction

[0036] see figure 1 , the neuronal antigen spectrum antibody detection kit according to an embodiment of the present invention, comprising an antigen-coated detection membrane strip, an enzyme conjugate, a sample buffer, a washing buffer and a substrate solution.

[0037] Wherein, the antigens coated in parallel on the detection membrane strip include Amphiphysin, CV2, PNMA2 (Ma2 / Ta), Ri, Yo and Hu. The width of the detection film strip is 2.5mm, and the functional area at the bottom of the strip is equipped with a quality control strip that can indicate whether the experimental results are reliable.

[0038] The enzyme conjugate was concentrated 10 times, and its pH value was 7.05. Each liter of enzyme conjugate included Tris 6.057g, sodium chloride 9g, BSA 25g, sucrose 15g, Proclin 300 1ml, Brij 35 1g, acetaminophen 0.5 g, gentamicin sulfate 0.04g, Tween 20 0.5ml, AP enzyme goat anti-human 0.8mg / L.

[0039] Enzyme conjugates should be diluted with sample buffer and used wi...

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Abstract

The invention discloses a neuron antigen spectrum antibody detection kit and a detection method thereof. The neuron antigen spectrum antibody detection kit comprises an antigen-coated detection membrane strip, an enzyme conjugate, a sample buffer solution, a cleaning buffer solution and a substrate solution. And the detection membrane strip is coated with antigens Amphiphysin, CV2, PNMA2 (Ma2 / Ta), Ri, Yo and Hu in parallel. The enzyme conjugate is one or more of alkaline phosphatase labeled anti-human immune globulins G (IgG), IgA (Immunoglobulin A) and IgM (Immunoglobulin M). The detection method is an immunoblotting method. The detection kit provided by the invention can be used for conveniently and quickly performing in-vitro disease diagnosis, and is simple to operate and high in sensitivity; and more diagnosis means are provided for the medical research field of nervous system lesions.

Description

technical field [0001] The invention relates to the field of kits, in particular to a neuron antigen spectrum antibody detection kit and a detection method thereof. Background technique [0002] Amphiphysin, CV2, PNMA2(Ma2 / Ta), Ri, Yo or Hu are neuron-associated antigens. In a series of neurological diseases, the body can produce autoantibodies against these antigens, leading to a series of neurological diseases or lesions , Such as sensitive neuritis, brainstem encephalitis, cerebellar encephalitis and limbic encephalitis and PNS. [0003] The anti-Amphiphysin (128kDa) antibody uses Amphiphysin as the target antigen, and Amphiphysin participates in the process of vesicle endocytosis. Therefore, detection of anti-Amphiphysin antibody against Eaton-Lambert myasthenic syndrome, autonomic, susceptibility, sensory motor polyneuropathy, ankylosing encephalomyelitis, cerebellar syndrome, sensory neuropathy, ocular myoclonic ataxia, stiff man Diagnosis of neurologic syndromes suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/6893G01N33/535G01N2800/28
Inventor 陶瑞谢红梅陈吉帅
Owner 苏州邦器生物技术有限公司