Efficient nitrogen barley screening method based on chlorate sensitivity

Abstract

The invention discloses a chlorate sensitivity-based nitrogen-efficient barley screening method, which comprises the following steps: revealing the relationship between chlorate and each index of nitrogen-efficient barley through research, determining a chlorate screening system and evaluation indexes suitable for nitrogen-efficient barley, and utilizing seedling height and overground part dry weight as screening indexes of nitrogen-efficient barley. The barley variety of which the seedling height and the overground part are sensitive to chlorate at the same time is defined as nitrogen-efficient barley, the nitrogen absorption efficiency can be rapidly distinguished in the early stage of barley, the identification time is saved, and the identification efficiency is improved; the method can be used for large-scale screening and identification, is obvious in phenotype and higher in accuracy, avoids inaccuracy of a single index, is good in repeatability, and is suitable for large-scale material identification.

Description

technical field [0001] The invention belongs to the technical field of barley breeding, and in particular relates to a nitrogen-efficient barley screening method based on chlorate sensitivity. Background technique [0002] Nitrogen is one of the macroelements necessary for plant growth and development, which affects the yield and quality of crops. Therefore, in agricultural production, a large amount of nitrogen fertilizer is usually applied to ensure maximum crop yield. However, the effective utilization rate of nitrogen fertilizer does not exceed 50%, and most of the nitrogen fertilizer is eventually lost to the soil and water, causing many environmental problems, such as soil acidification, water eutrophication, global warming and so on. Therefore, carrying out crop nitrogen-efficient breeding can increase crop yield while reducing the use of nitrogen fertilizer, which is an ideal way for the current sustainable agricultural development. [0003] Barley (Hordeum vulgare...

AI Technical Summary

Problems solved by technology

[0005] At present, there are problems with these screening methods: 1) Long cycle, time-consuming and laborious, large environmental impact, poor repeatability, and difficult to carry out on a large scale; 2) There is no uniform standard for the concentration and treatment time of low nitrogen stress, and the relative value cannot be counted Analysis, it is difficult to distinguish the data near the critical value or the materials with small differences; 3) It is not suitable for large sample screening

Although this criterion based on chlorate treatment can screen and identify nitrogen-efficient barley materials, it is often subjective to a certain extent in judging by leaf color, and the actual estimation is relatively difficult, especially it is difficult to distinguish materials with small differences , cannot accurately classify nitrogen-efficient barley

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