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Method for producing pogostemon cablin seedlings through stem tip detoxification

A technology for detoxifying and patchouli from shoot tips, which is applied in the field of shoot tip detoxification to produce patchouli seedlings, can solve the problem that the large-scale and industrialized production of patchouli seedlings cannot be satisfied, and the resistance of patchouli to diseases and insect pests cannot be satisfied. Problems such as variation, germplasm cannot be mutated or evolved, etc., to achieve the effect of simple and convenient configuration method and operation, improved planting level, and good economic benefits

Inactive Publication Date: 2022-05-13
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Patchouli rarely blooms in my country, and even if it does, it is difficult to produce fruit. Therefore, patchouli can only be propagated by cuttings or tissue culture, and its germplasm cannot mutate or evolve through sexual reproduction.
Long-term asexual reproduction makes pathogens accumulate and spread from generation to generation, resulting in poor resistance of patchouli to diseases and insect pests, decreased yield and quality
Although the traditional method of tissue culture propagation of Patchouli seedlings overcomes the problem of low propagation coefficient in cutting propagation, there is no detoxification process, the seed property is not fully restored, and the seedling stage is weak and the production cost is too high. The effect of purification and rejuvenation has not been reached, and the needs of large-scale and industrialized production of patchouli seedlings cannot be met.

Method used

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  • Method for producing pogostemon cablin seedlings through stem tip detoxification
  • Method for producing pogostemon cablin seedlings through stem tip detoxification
  • Method for producing pogostemon cablin seedlings through stem tip detoxification

Examples

Experimental program
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Effect test

Embodiment 1

[0065] A method for producing healthy seedlings of Patchouli by using stem tip detoxification, comprising the following steps:

[0066] (1) Selection and disinfection of explants:

[0067] ①Select the stem section with buds at the top of Shipai patchouli that grows healthy and has no pests and diseases as explants;

[0068] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, and then remove the excess leaves; the neutral detergent can be a saturated washing powder solution.

[0069] ③ On the ultra-clean workbench, pre-treat once with sterile water, soak in 70% ethanol for 20 sec, and then rinse twice with sterile water for 3 minutes each time;

[0070] ④ Immediately transfer to 10% sodium hypochlorite solution for disinfection for 13 minutes, and finally wash with sterile water 4 times for 4 minutes each time to obtain sterilized explants.

[0071] (2) Shoot tip stripping and callus induction:

[0072] Remove the excess stem segme...

Embodiment 2

[0096] A method for producing healthy seedlings of Patchouli by using stem tip detoxification, comprising the following steps:

[0097] (1) Selection and disinfection of explants:

[0098] ①Select the stem segment with buds at the top of Patchouli Gaoyao that grows healthy and has no pests and diseases as explants;

[0099] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, and then remove the excess leaves;

[0100] ③ On the ultra-clean workbench, pre-treat once with sterile water, soak in 70% ethanol for 10 sec, and rinse twice with sterile water for 3 minutes each time;

[0101] ④ Immediately transfer to 10% sodium hypochlorite solution for disinfection for 15 minutes, and finally wash with sterile water 3 times, each time for 3 minutes, to obtain sterilized explants.

[0102] (2) Shoot tip stripping and callus induction:

[0103] Remove the excess stem segment from the sterilized explant obtained in step (1), and keep the ste...

Embodiment 3

[0127] A method for producing healthy seedlings of Patchouli by using stem tip detoxification, comprising the following steps:

[0128] (1) Selection and disinfection of explants:

[0129] ① Select the stem section with buds at the top of Patchouli Zhanjiang that grows healthy and has no pests and diseases as explants;

[0130] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, and then remove the excess leaves;

[0131] ③ On the ultra-clean workbench, pre-treat once with sterile water, soak in 70% ethanol for 30 sec, and then rinse twice with sterile water for 3 minutes each time;

[0132] ④ Immediately transfer to 10% sodium hypochlorite solution for disinfection for 10 minutes, and finally wash 5 times with sterile water for 5 minutes each time to obtain sterilized explants.

[0133] (2) Shoot tip stripping and callus induction:

[0134] Remove the excess stem segment from the sterilized explant obtained in step (1), and keep ...

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Abstract

The invention discloses a method for producing pogostemon cablin seedlings through stem tip detoxification, which comprises the following steps of: establishing a stem tip culture detoxification method according to the breeding and growth characteristics of the pogostemon cablin seedlings; the culture medium suitable for callus induction, proliferation and aseptic seedling rooting in the pogostemon cablin tissue culture process is developed. In addition, a molecular detection method for germs in pogostemon cablin seedlings is also established, and sterile seedlings are obtained; by means of the breeding mode of first-generation virus-free seedlings, second-generation virus-free seedlings and production seeds, the breeding coefficient is increased, and healthy pogostemon cablin seedlings can be produced all the year round. By using the culture medium for callus induction, the culture medium for proliferation and the culture medium for rooting, the propagation coefficient can be effectively increased, and the production cost can be reduced; by using a three-generation virus-free seedling breeding mode, the production period can be shortened, and the planting benefit is greatly improved; the problems that pogostemon cablin carries poison and the propagation coefficient is low can be effectively solved, and the established pogostemon cablin healthy seedling propagation system also provides good economic benefits for planters.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for producing patchouli seedlings by detoxifying stem tips. Background technique [0002] Patchouli Pogostemon cablin (Blanco) Benth. belongs to Labiatae Patchouli, native to the Philippines, Malaysia, Vietnam and other Southeast Asian countries. The whole herb of Patchouli is used as medicine, and has always been regarded as a good medicine for summer and damp seasons. It is one of the famous "Top Ten Cantonese Medicines". In addition, the patchouli oil extracted from it is also widely used in medicine and light chemical industry, and can be used to prepare various ointments, pills, cosmetic skin care products, fixatives and insecticides. Patchouli can be used as both medicine and spice, and has a very broad application prospect. [0003] Patchouli rarely blooms in my country, and even if it does, it is difficult to produce fruit. Theref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/10A01G22/00
CPCA01H4/008A01H4/002A01G2/10A01G22/00
Inventor 王继华周芳顾艳梅瑜蔡时可徐世强孙铭阳李静宇
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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