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Hair follicle stem cell (ORSC) preparation and application thereof in nerve injury

A technology of hair follicle stem cells and preparations, applied in animal cells, cell dissociation methods, nervous system diseases, etc., can solve problems such as poor therapeutic effect, difficulty in achieving expected results, and differences in hair follicle stem cells, so as to maintain survival rate and increase survival time And the differentiation rate, the effect of increasing the differentiation rate

Pending Publication Date: 2022-05-13
浙江卫未生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the injection of cultured hair follicle stem cells is mostly used to treat neurological injury, and the drug is administered to the nerve injury site, but the therapeutic effect of this method is poor: on the one hand, the cells injected into the body after culture are easily eliminated by the body, and on the other hand On the one hand, different patients will have significant differences in hair follicle stem cells, and it is difficult to achieve the expected effect

Method used

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  • Hair follicle stem cell (ORSC) preparation and application thereof in nerve injury
  • Hair follicle stem cell (ORSC) preparation and application thereof in nerve injury
  • Hair follicle stem cell (ORSC) preparation and application thereof in nerve injury

Examples

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Embodiment 1

[0045] A hair follicle stem cell (ORSC) preparation, including components and concentrations: PBS buffer, hair follicle stem cells, trehalose solution, compound differentiation inducer, growth factor, proliferation inhibitor;

[0046]

[0047] A preparation method of hair follicle stem cell (ORSC) preparation, comprising steps:

[0048] S11. Wash the human skin tissue containing hair follicles repeatedly with PBS solution for 2-3 times to remove excess blood and impurities attached to the human skin tissue, and then use sterile surgical scissors to cut the scalp tissue into 2mm along the direction of the hair shaft* 5mm leather strips to obtain clean human skin tissue;

[0049] S12. Using a two-step enzyme digestion method, put the strip-shaped tissue obtained in step S11 into a solution containing 0.25% neutral protease, digest the digestive solution in 1% double antibody at 4°C overnight, and add the scalp tissue containing Wash the double antibody in a normal saline cul...

Embodiment 2

[0058] Prepare this hair follicle stem cell (ORSC) preparation according to the preparation method described in Example 1, and the components and concentrations are as follows:

[0059]

Embodiment 3

[0061] Prepare this hair follicle stem cell (ORSC) preparation according to the preparation method described in Example 1, and the components and concentrations are as follows:

[0062]

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PUM

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Abstract

The invention provides a hair follicle stem cell (ORSC) preparation and application thereof in nerve injury, the hair follicle stem cell (ORSC) preparation comprises a PBS buffer solution, hair follicle stem cells, a trehalose solution, a composite differentiation inducer, a growth factor and a proliferation inhibitor. The invention relates to a hair follicle stem cell (ORSC) preparation, which is added with a trehalose solution, a composite differentiation inducer, a growth factor and a proliferation inhibitor: the trehalose solution forms a protective film on the cell surface, and is used for keeping the survival rate of hair follicle stem cells in a life body environment, and the hair follicle stem cells are not easy to remove by a life body after being injected; the compound differentiation inducer and the growth factor are matched for directionally differentiating the hair follicle stem cells into nerve cells; the proliferation inhibitor plays a role in inhibiting proliferation of common cells at the damaged part by inhibiting the progress of a cell cycle, and the differentiation rate of hair follicle stem cells is increased.

Description

technical field [0001] The invention relates to a hair follicle stem cell (ORSC) preparation and its application in nerve injury, belonging to the technical field of stem cell preparations. Background technique [0002] There are about five million hair follicles in the human body. The hair follicle is a miniature organ composed of various cells. It is divided into hair shaft, inner root sheath and outer root sheath from the inside to the outside. The lower end of the hair follicle expands into a hair bulb to accommodate the hair papilla. Hair follicles grow periodically, which is divided into growth phase, degenerative phase and resting phase in turn. The growth of new hair requires the cells located in the outer root sheath of the hair follicle, that is, the bulge area, to enter the growth phase, and the adjacent mesenchymal dermal papilla cells assist and Hair buds are formed with the participation of the stem cell microenvironment. [0003] Hair follicle stem cells exis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K35/36A61P25/00C12N5/0775A61K31/7076A61K31/7004A61K33/24
CPCA61K35/36A61K38/185A61K38/1825A61K31/7076A61K31/7004A61K33/24A61P25/00C12N5/0666C12N2509/00A61K2300/00
Inventor 陈锦阳
Owner 浙江卫未生物医药科技有限公司