Bacillus coagulans and application thereof in corn soaking

A Bacillus coagulans and corn soaking technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high usage, long soaking time, high energy consumption, etc., and achieve reduced sulfur dioxide consumption and soaking effect Good, high starch yield effect

Pending Publication Date: 2022-05-13
JILIN COFCO BIOCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is in order to overcome SO in the corn steeping process that prior art exists 2 Problems such as high usage, long soaking time, and high energy consumption provide a strain of Bacillus coagulans and its application in corn soaking. When soaking corn with bac

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  • Bacillus coagulans and application thereof in corn soaking
  • Bacillus coagulans and application thereof in corn soaking
  • Bacillus coagulans and application thereof in corn soaking

Examples

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Example Embodiment

[0050] Example 1

[0051] The present embodiment is used to illustrate the isolation, purification and preservation of Bacillus coagulans CGMCC No.23993 provided by the present invention.

[0052] (1) Strain isolation and purification

[0053] The inventor of the present invention obtained a strain of Bacillus coagulans CGMCC No.23993 isolated from the corn soaking solution used in the countercurrent soaking process of corn starch production line of COFCO Biochemical Energy (Elm) Co., Ltd. The specific separation and purification process is as follows:

[0054] Corn syrup dry powder liquid medium was used as the enrichment medium, and its composition was: glucose 20g / L, beef paste 10g / L, peptone 10g / L, yeast paste 5g / L, triammonium citrate 2g / L, sodium acetate 5g / L, dipotassium phosphate 2g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L.

[0055] Take COFCO Biochemical Energy (Yushu) Co., Ltd., 0.5mL of corn soaking solution when corn is soaked for 6h in the counter-current...

Example Embodiment

[0059] Example 2

[0060] The present embodiment is intended to illustrate the improvement of the corn soaking effect of Bacillus coagulans CGMCC No.23993 provided by the present invention.

[0061] Bacillus coagulans DUT21 was cultured with corn syrup dry powder medium, and the specific culture conditions included: 50±2 °C temperature, 200 rpm shaker for 24 hours, and DUT21 culture solution was obtained for backup. The OD of this DUT21 culture solution was tested 600 Values are approximately 6.1±0.7 (equivalent to DUT21) of approximately 6.08 ×10 8 CFU / mL)。

[0062] (1) Effect of Bacillus coagulans DUT21 inoculation on maize soaking effect

[0063]The amount of inoculation using DUT21 culture solution was 4 volume, 6 volume, 8 volume, 10 volume, 12 volume, and the total volume was 150 mL (the remaining amount was replenished with sterile water) of corn soaking solution. A certain amount of SO is introduced into this soaking solution 2 , so that the soaking solution is in SO 2 Th...

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Abstract

The invention relates to the technical field of corn deep processing, and particularly discloses bacillus coagulans and application thereof in corn soaking, and the preservation number of the bacillus coagulans is CGMCC (China General Microbiological Culture Collection Center) No.23993. The bacillus coagulans provided by the invention has the characteristics of acid resistance, high temperature resistance and the like, and when the bacillus coagulans is used for soaking corn, the yield of corn starch can be remarkably increased, the soaking time can be shortened, and the usage amount of SO2 can be reduced. Besides, when the bacillus coagulans provided by the invention is used for soaking the corn, corn kernels do not need to be crushed, and the corn can be soaked by adopting a one-step method, so that the process is simplified, and the bacillus coagulans is more suitable for industrial production and application.

Description

technical field [0001] The invention relates to the technical field of corn deep processing, in particular to a strain of bacillus coagulans and its application in corn soaking. Background technique [0002] The production of corn starch is mainly divided into dry method and wet method, among which the wet method is a kind of starch preparation process widely used at present. In the wet process, after soaking the corn kernels, they are coarsely and finely ground to separate the germ, fiber and protein, and finally obtain high-purity cornstarch. Soaking is the first and most important process in the wet process of corn starch production. The effect of soaking has a significant impact on the yield of corn starch. [0003] The purpose of soaking is to soften the corn kernels, destroy or weaken the binding force between protein and starch granules, and free the starch. At present, the conventional corn soaking process usually needs to add a higher concentration of SO to the so...

Claims

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Application Information

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IPC IPC(8): C12N1/20C08B30/02C12R1/07
CPCC12N1/20C08B30/02
Inventor 佟毅孙亚琴夏文静修志龙赵雪松李义金渭武王小艳
Owner JILIN COFCO BIOCHEM
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