Serum type 1 Marek's disease virus MEQ monoclonal antibody, and preparation method and application thereof

Abstract

The invention discloses an anti-serum type 1 Marek's disease virus MEQ protein monoclonal antibody as well as a preparation method and application thereof. The anti-MDV-1 specific MEQ protein monoclonal antibody is generated by a mouse monoclonal antibody hybridoma cell strain meq-2A9-B12, the cell strain is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No: 23023, the subtype of the monoclonal antibody is IgG2b, the light chain type of the monoclonal antibody is Kappa, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.3, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.4. The monoclonal antibody can be used for detecting the MDV-1 specific MEQ protein. And the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.4. The monoclonal antibody 2A9-B12 secreted by the invention can be used for immunological detection analysis of IFA, Western blot and the like of strains with different pathotypes of MDV-1, can also be used for identifying and distinguishing MDV-2 and MDV-3 strains, and is suitable for differential diagnosis of MDV epidemic strains with different serotypes in clinical suspected cases. The invention solves the problem of'neck 'of lack of anti-MDV-1 MEQ protein specific monoclonal antibody in China at present, and provides a key core reagent for subsequent research of MD and research and development of diagnostic reagents.

Description

technical field [0001] The invention relates to a monoclonal antibody against serotype 1 Marek's disease virus MEQ, a preparation method and application thereof, and belongs to the technical field of animal immunology. Background technique [0002] Chicken Marek's disease (Marek's disease, MD) is an important immunosuppressive disease and tumor disease caused by early infection of chickens by Marek's disease virus (MDV). Severe immunosuppression after MDV infects the host in the early stage is one of the important factors for the poor immune prevention effect of clinical poultry disease vaccines. It has long plagued the healthy development of my country's poultry industry. At the same time, MDV will induce visceral tumors and a large number of deaths after infecting natural hosts, resulting in an average annual direct economic loss of about US$1-2 billion in the global poultry industry. The occurrence of MDV-induced host tumors can be effectively prevented by vaccines. It i...

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Problems solved by technology

[0007]Although domestic scholars have tried many times to develop antibodies to this protein, due to factors such as the high GC content of the meq gene sequence, the large protein size, and the difficulty in artificial expression and purification , my country has not yet been able to develop a practical MEQ antibody, and there are no commercial products available on the market

At present, only the World Organization for Animal Health (OIE) MDV Reference Laboratory of the United States Department of Agriculture's Poultry Disease and Tumor Laboratory and the British Pirbright Institute have successfully developed a monoclonal antibody for this protein, but no commercial use has been made. Chinese scholars carry out relevant research, and the antibodies needed can only rely on the friendship gifts of European and American scholars from these two institutions, which greatly limits the research needs of Chinese scholars

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