Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibacterial peptide YHX-5 and application thereof

A YHX-5, antibacterial peptide technology, applied in applications, antibacterial drugs, peptides, etc., to achieve the effect of saving large-scale production costs, short synthetic sequences, and small molecular weights

Active Publication Date: 2022-05-20
OCEAN UNIV OF CHINA
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned problems that the existing antimicrobial peptides cannot satisfy the clinical application, the present invention provides an antimicrobial peptide YHX-5 and its application. Salmonella have good antibacterial activity, and the hemolytic activity is very low, the safety is very high, the synthetic sequence is short, and the chemical synthesis is not difficult

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial peptide YHX-5 and application thereof
  • Antibacterial peptide YHX-5 and application thereof
  • Antibacterial peptide YHX-5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The design of embodiment 1 antimicrobial peptide

[0029] Based on the de novo design of antimicrobial peptides and the understanding of the structure-activity relationship, the sequence parameters of 843 antibacterial peptide sequences screened from the ADP3 database with antibacterial effects on Gram-positive and Gram-negative bacteria were analyzed (including Sequence length, charged number, hydrophobic amino acid ratio and amino acid composition), and then according to the selection principle of the dominant parameters and combined with rational design ideas, the sequence parameters of the new antimicrobial peptide were determined, as follows:

[0030] Table 1 Design parameters of antimicrobial peptide sequences

[0031]

[0032] Select 13, which ranks second in the parameter frequency in the database, as the sequence length of the new antimicrobial peptide, which can effectively reduce the synthesis cost while reducing the molecular weight and improving safety. ...

Embodiment 2

[0042] Embodiment 2 antibacterial activity

[0043] Antimicrobial peptides were synthesized by Qiangyao Biotechnology (Shanghai) Co., Ltd.

[0044] Staphylococcus aureus, Escherichia coli and Salmonella were respectively inoculated on LB solid medium, Listeria monocytogenes and Streptococcus mutans were inoculated on BHI solid medium, and cultured in a constant temperature incubator at 37°C After 18 hours, a single colony of each strain was picked and placed in their corresponding liquid medium, and cultured with shaking at a constant temperature of 37°C for 12 hours. Measure the OD of the bacterial solution 600 value, and dilute it to 1×10 6 CFU / mL.

[0045] ①Inhibition zone test

[0046] Prepare LB and BHI semi-solid medium (mass fraction of agar 0.6%), add 7 μL of bacterial solution to 20mL of each plate, shake and mix well, pour it into the petri dish with Oxford cup placed, and pull out the Oxford cup after the medium is cooled and solidified to complete the infusion....

Embodiment 3

[0051] Example 3 Hemolytic activity

[0052] Take 1mL of fresh healthy rabbit blood and add it to a heparin anticoagulant tube, centrifuge at 1000xg for 10min, get the precipitate, wash it with PBS buffer 3 times, and resuspend the red blood cells in 10mL of PBS. Adjust the concentration of antimicrobial peptide YHX-5 to 4-512 μg / mL with PBS buffer, add 50 μL per well into a 96-well plate, and add an equal volume (50 μL) of red blood cell suspension and mix well. Take PBS buffer as a negative control, and 0.1% Tritonx-100 as a negative control, take it out after incubating at 37°C for 1 hour, centrifuge at 1000xg for 10 minutes, take out the supernatant and measure the OD value at 570nm with a microplate reader, and the test results are shown in the table 4.

[0053] The calculation formula of hemolysis rate is: hemolysis rate=(A T -A O ) / (A C -A O )×100%.

[0054] In the formula: A T is the absorbance value of the experimental group, A C is the absorbance value of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology. Aiming at the problem that the existing antibacterial peptide cannot meet the clinical application, the invention provides the antibacterial peptide YHX-5 and the application thereof. The amino acid sequence of the antibacterial peptide YHX-5 is as follows: Gly-Lys-Leu-Leu-Ser-Lys-Leu-Leu-Gly-Lys-Leu-Leu-Lys-NH2, and the amino acid sequence of the antibacterial peptide YHX-5 is as follows: the amino acid sequence of the antibacterial peptide YHX-5 is as follows: Gly-Lys-Leu-Leu-Lys-NH2. The antibacterial peptide disclosed by the invention has relatively good antibacterial activity on listeria monocytogenes, streptococcus mutans, escherichia coli and salmonella. The antibacterial peptide disclosed by the invention is very low in hemolytic activity, very high in safety, short in synthetic sequence and low in chemical synthesis difficulty.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an antibacterial peptide and its application. Background technique [0002] Antibiotics are undoubtedly one of the greatest discoveries in the history of human medicine and have saved tens of millions of lives. The antibacterial mechanism of antibiotics mainly includes four aspects: inhibiting the synthesis of bacterial cell walls, enhancing the permeability of bacterial cell membranes, interfering with bacterial protein synthesis and inhibiting the process of nucleic acid replication and transcription. However, in recent years, the abuse of antibiotics has led to the continuous increase of drug-resistant strains and the emergence of multi-drug-resistant bacteria, which pose a serious threat to the treatment of clinical infections. Therefore, it is urgent to solve the problem of drug resistance of pathogenic bacteria. The development of new antibacterial drugs is an imp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A23K20/147A23L3/3526A61K8/64A61Q19/00A61Q19/10A61Q17/00A61Q11/00A61Q5/02A01N37/46A01P1/00
CPCC07K7/08A61P31/04A23K20/147A23L3/3526A61K8/64A61Q19/00A61Q19/10A61Q17/005A61Q11/00A61Q5/02A01N37/46A61K38/00A23V2002/00A61K2800/10A61K2800/524A23V2200/10A23V2250/55Y02A50/30
Inventor 易华西李剑勋刘银雪刘伊索步雨珊刘同杰公丕民张兰威
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com