Culture medium for culturing recurrent bladder cancer biopsy sample organoid and culture method thereof

A culture medium, bladder cancer technology, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of low success rate of culture, obstacles to precise treatment of bladder cancer, etc., and achieve the effect of stable and reliable source and low cost

Inactive Publication Date: 2022-05-20
上海诺典生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This poses an obvious obstacle to tumor organoids to guide precision therapy for recurrent bladder cancer
[0006] The technical problem to be solved in the present invention is to develop a variety of culture media with different components aimed at the high heterogeneity of recurrent bladder cancer on the basis of fully understanding the molecular biology of recurrent bladder cancer and the rich experience in organoid culture in the past. Solved the problem of low success rate of recurrent bladder cancer tumor organoid culture, and laid a solid foundation for tumor organoids to guide the precise treatment of recurrent bladder cancer

Method used

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  • Culture medium for culturing recurrent bladder cancer biopsy sample organoid and culture method thereof
  • Culture medium for culturing recurrent bladder cancer biopsy sample organoid and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, improved culture medium 1

[0056] DMEM / F12K medium (1:1), ascorbic acid (10mg / L), L-glutamic acid (50mg / L), sodium selenite (0.05mg / L), ethanolamine hydrochloride (20mg / L), Ammonium metavanadate (5×10 -4 mg / L), manganese chloride tetrahydrate (7×10 -5 mg / L), R-spondin 1 (200ng / ml), Noggin (20ng / ml), N2:B27 (1:1), glutamine supplement (1×), HEPES (1×), nicotinamide ( 20mM), Normocin TM (1×), N-acetylcysteine ​​(2 mM), A83-01 (10 μM), EGF (100 ng / ml), FGF10 (20 ng / ml), heparin (100 IU / ml).

Embodiment 2

[0057] Embodiment 2, improved culture medium 2.

[0058] DMEM / F12K medium (2:1), ascorbic acid (2mg / L), L-glutamic acid (100mg / L), sodium selenite (0.1mg / L), ethanolamine hydrochloride (50mg / L), Ammonium metavanadate (20×10 -4 mg / L), manganese chloride tetrahydrate (15×10 -5 mg / L); R-spondin 1 (500ng / ml), Noggin (50ng / ml), N2:B27 (1:2), glutamine supplement (1×), HEPES (1×), nicotinamide ( 5mM), Normocin TM (1×), N-acetylcysteine ​​(2 mM), HGF (100 ng / ml), DMSO (1%, v / v), CHIR99021 (50 μM).

Embodiment 3

[0059] Example 3. Comparison of recurrent bladder cancer organoids cultured in two improved media and primary bladder cancer (PNAS)

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Abstract

The invention discloses a culture medium for a recurrent bladder cancer organ and a culture method of the culture medium. By adjusting the content of each component in the culture medium, the organoid culture of the high-heterogeneity recurrent bladder cancer can be realized, the success rate of the organoid culture is improved, and a foundation is laid for screening specific anti-cancer drugs.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a medium for recurrent bladder cancer organoids and a culture method thereof. Background technique [0002] Bladder cancer is an important tumor that affects human health and survival and increases the economic burden of residents. At present, the number of new cases of bladder cancer worldwide is about 570,000 cases per year, ranking 12th among all tumors; the number of deaths due to bladder cancer is about 210,000 cases worldwide every year, ranking 14th among all tumors. The 5-year survival rate of bladder cancer patients after comprehensive treatment by various means is as high as 77%, and the 5-year survival rates of bladder cancer in situ, local, regional and distant metastasis patients are 96%, 69%, 37% and 6% respectively. %. The most important factor affecting the survival and prognosis of bladder cancer patients is tumor recurrence after initial treatment, and the recurren...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N5/09C12N5/071C12Q1/02
CPCC12N5/0693C12N5/0684G01N33/5011G01N33/5044C12N2500/38C12N2500/32C12N2500/05C12N2500/46C12N2500/20C12N2501/11C12N2501/119C12N2501/91C12N2501/415C12N2501/12C12N2500/62C12N2503/02G01N2500/10
Inventor柏卫华
Owner上海诺典生物科技有限公司