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Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood

A growth factor and autologous blood technology, applied in infusion sets, centrifuges, medical science, etc., can solve problems such as the inability to prepare solid and liquid CGF at the same time, achieve high market application prospects, increase the number and efficiency of preparation, and avoid cross-infection Effect

Pending Publication Date: 2022-05-27
无锡创盟精准医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention proposes a novel method for preparing solid or liquid naturally concentrated growth factor CGF from autologous blood aiming at the problem that traditional domestic centrifuges cannot prepare solid and liquid CGF at the same time

Method used

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  • Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood
  • Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood
  • Method for preparing solid or liquid natural concentrated growth factor CGF from autoblood

Examples

Experimental program
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Effect test

Embodiment 1

[0024] like figure 1 and image 3 As shown, this example provides a solid CGF preparation process, and the product is as figure 1 .

[0025] (1) Preparation of reagent consumables: prepare blood collection needles, 2 disposable 5 ml syringes, 1 pulse pressure belt, 2 disposable sterile dressing bags, 2 red sterile disposable vacuum blood collection tubes (EDTK2 test tubes), Two disposable lancets, no additives.

[0026] (2) Blood collection: place the donor's arm on the table with the palm facing upwards, and the nurse will place a small cushion under the arm. At this time, the arm should be placed at the same height as the height of the heart. At this time, the nurse ties a tourniquet, disinfection and venipuncture at the elbow. The fist should be clenched when the blood is drawn, and the fist can be released after the needle enters the blood vessel. The patient should keep a quiet and stable state of mind at this time. If he is nervous about venipuncture or has blood na...

Embodiment 2

[0032] This embodiment provides the liquid CGF production process, and the product after centrifugation is as follows: figure 2 . , where PPP and between PRP layers and The CGF layer is formed between PRP, CD34+ layer, CD34+ and red blood cells.

[0033] Prepare cuffs, sterile alcohol cotton balls, purple anti-coagulation test tubes, and disposable blood collection bags. According to the blood drawing method of Example 1, the required blood collection volume was estimated. Draw 10ml of venous blood into the anticoagulation tube, immediately extract the mixed anticoagulant from the blood collection bag according to the ratio of 0.3ml / 10ml, put it into the special purple anticoagulation tube and shake it for five or six times. Put the test tube into the special centrifuge tube, balance the centrifuge and cover it.

[0034] Centrifuge at 2700 r / min for 2 min, 2400 r / min for 4 min, 2700 r / min for 4 min, and 3000 r / min for 3 min. After 13 min of continuous variable speed c...

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Abstract

The invention belongs to the technical field of natural concentrated growth factor CGF preparation, and relates to a method for preparing a solid or liquid natural concentrated growth factor CGF from autoblood. The method comprises the following steps: collecting blood, respectively injecting the blood into a purple sterile anti-coagulation test tube and a sterile vacuum red blood collection tube, adding a mixed anti-coagulation agent into the purple sterile anti-coagulation test tube, uniformly shaking, centrifuging, and taking a middle CGF layer to obtain liquid CGF; centrifuging the sterile vacuum red blood collection tube, taking the middle layer and the gel at the junction of the middle layer and the lower layer, and pressing and shaping by a film pressing device to obtain the solid CGF. The CGF preparation amount is large, the operation is convenient, the practicability is high, and the market application prospect is relatively high.

Description

technical field [0001] The invention belongs to the technical field of natural concentrated growth factor CGF preparation, and relates to a method for preparing solid or liquid natural concentrated growth factor CGF from autologous blood. Background technique [0002] The study of plasma extracts has roughly gone through three stages: Platelet-rich plasma (PRP), Platelet-rich fibrin (PRF) and Concentrate Growth Factors (CGF). Because PRP contains thrombin and animal serum, and adopts a centrifugation method, it is easily contaminated and sensitized, and is facing elimination. Compared with CGF, the results of scanning electron microscopy showed that collagen fibers with different diameters were arranged in the upper part of platelet-rich fibrin and concentrated growth factors to form a three-dimensional network structure, and a large number of granular platelets were attached to the collagen fibers, and there was no cell structure; platelet-rich Collagen fibers in fibrin ar...

Claims

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Application Information

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IPC IPC(8): A61B5/153A61M5/178B04B5/04
CPCA61B5/153A61B5/702A61M5/178B04B5/0414
Inventor 谭晓雯谭汇泉
Owner 无锡创盟精准医学科技有限公司
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