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Tomato bacterial wilt antagonistic strain and application thereof in prevention and treatment of tomato bacterial wilt

A technology of tomato bacterial wilt and bacterial strains, applied in the field of tomato bacterial wilt antagonistic strains and its application in the control of tomato bacterial wilt, can solve the problems of unstable control effect and achieve good biocontrol potential effect

Active Publication Date: 2022-05-31
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The antagonistic strains screened out by predecessors have obvious effects in indoor pot experiments, but the control effect is not stable under field conditions

Method used

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  • Tomato bacterial wilt antagonistic strain and application thereof in prevention and treatment of tomato bacterial wilt
  • Tomato bacterial wilt antagonistic strain and application thereof in prevention and treatment of tomato bacterial wilt
  • Tomato bacterial wilt antagonistic strain and application thereof in prevention and treatment of tomato bacterial wilt

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Embodiment Construction

[0025] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0026] Screening and identification of antagonistic bacteria against tomato bacterial wilt

[0027] 1. Materials

[0028] 1.1 Soil samples for testing The soil samples were collected from the rhizosphere soil of healthy tomato plants in Xianggu Village, Fancun Town, Taigu County, and a total of 38 soil samples were collected.

[0029] 1.2 The test strain Tomato solanacearum ( Ralstonia solanacearum ), provided by the Plant Pathology Laboratory of Shanxi Agricultural University.

[0030] 1.3 Test medium NA medium: 10 g sucrose, 5 g peptone, 3 g beef extract, 15 g agar powder, 1000 mL distilled water, pH 7.0; NB medium without agar powder in N...

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Abstract

The invention relates to the field of biocontrol bacteria, in particular to a tomato bacterial wilt antagonistic strain and application thereof in prevention and treatment of tomato bacterial wilt, the antagonistic strain is Pseudomonas aeruginosa B-6, the 16S rDNA sequence of the antagonistic strain is as shown in SEQ ID NO: 1, the antagonistic strain is preserved in China General Microbiological Culture Collection Center on December 7, 2021, and the preservation number is CGMCC No: 24048; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The antagonistic strain B-6 provided by the invention has good biocontrol potential, and can be developed into a biocontrol inoculant with growth-promoting and disease-resisting effects.

Description

technical field [0001] The invention relates to the field of biocontrol bacteria, in particular to a tomato bacterial wilt antagonistic strain and its application in preventing and treating tomato bacterial wilt. Background technique [0002] Tomato bacterial wilt is a typical soil-borne bacterial disease of Solanaceae vegetables, caused by R. solanacearum ( Ralstonia solanacearum ) infection caused by the disease all over the country. The disease can cause large-scale wilting or even death of infected crops, and the incidence of bacterial wilt can be as high as 80% or more in severely affected fields, seriously affecting the yield of tomatoes, and in severe cases resulting in failure of the harvest. When the tomato plant is about 30 cm tall, the bacterial wilt diseased plant begins to show symptoms, and the leaves will wilt and droop but remain green. Cut the base of the diseased plant stem, and the vascular bundles will turn brown. Generally, the fields enter the fruit...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P1/00A01P21/00C12R1/385
CPCC12N1/20A01N63/27Y02A40/10
Inventor 王美琴卢晓虹王戎杰许敏李甜爽刘海霞王春伟王燕郝晓娟姚艳平
Owner SHANXI AGRI UNIV