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Pseudomonas aeruginosa YZ66 and application of pseudomonas aeruginosa YZ66 in prevention and treatment of tobacco fusarium root rot and growth promotion

A technology of Pseudomonas aeruginosa, YZ66, applied in the field of microorganisms

Pending Publication Date: 2022-07-05
TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on the biocontrol bacteria of Fusarium tobacco root rot

Method used

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  • Pseudomonas aeruginosa YZ66 and application of pseudomonas aeruginosa YZ66 in prevention and treatment of tobacco fusarium root rot and growth promotion
  • Pseudomonas aeruginosa YZ66 and application of pseudomonas aeruginosa YZ66 in prevention and treatment of tobacco fusarium root rot and growth promotion
  • Pseudomonas aeruginosa YZ66 and application of pseudomonas aeruginosa YZ66 in prevention and treatment of tobacco fusarium root rot and growth promotion

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Experimental program
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Embodiment Construction

[0021] A strain of Pseudomonas aeruginosa YZ66, the specific operation steps for the screening and analysis of the described Pseudomonas aeruginosa YZ66 are as follows:

[0022] (1) Isolation and purification of bacterial strains from the root soil of healthy tobacco plants in Henan Henan and Henan West tobacco areas;

[0023] (2) Screening of antagonistic bacteria;

[0024] (3) Determination of antibacterial spectrum of antagonistic bacteria;

[0025] (4) Determination of antagonistic strains on tobacco seed germination and growth promotion;

[0026] (5) Determination of the antibacterial activity of the antagonistic bacteria fermentation filtrate;

[0027] (6) Determination of antibacterial activity of antagonistic volatile compounds;

[0028] (7) Identification of antagonistic bacteria;

[0029] (8) Detection of synthetic genes of antagonistic antibiotics;

[0030] (9) Determination of pot-plant control effect of antagonistic bacteria on Fusarium root rot and growth-pr...

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Abstract

The invention discloses application of pseudomonas aeruginosa YZ66 in prevention and treatment of tobacco fusarium root rot and growth promotion. The invention discloses pseudomonas aeruginosa YZ66 which has an obvious antagonistic effect on tobacco fusarium root rot, and the bacteriostatic mechanism and the growth promoting characteristic of the pseudomonas aeruginosa YZ66 are preliminarily analyzed; the bacterial strain has a wide antibacterial spectrum on main root and stem diseases of tobacco, has a relatively strong antibacterial effect on tobacco fusarium oxysporum and fusarium solani mainly by inhibiting spore germination and mycelial growth through antibiotics generated by thalli, can promote the growth and development of tobacco plants, and lays a foundation for subsequent research and application of field biological prevention and control.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Pseudomonas aeruginosa YZ66 and its application in preventing and treating Fusarium tobacco root rot and promoting growth. Background technique [0002] Tobacco Fusarium root rot is a fungal disease caused by Fusarium spp., F. oxysporum and F. solani are the dominant pathogens. It mainly invades from the roots of tobacco plants, resulting in blackened roots, yellow and wilted leaves, decay of vascular bundles, and finally the death of the entire plant, bringing serious disasters to tobacco production (Berruezo et al., 2018). In production, chemical agents are used for root transplanting and hole application. However, long-term application of chemical agents will cause drug resistance of pathogens and pesticide residues, which will negatively affect the ecological environment and human health (Li Ming et al., 2002). In recent years, the control of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/18C12Q1/04A01N63/27A01P3/00A01P21/00C12R1/385
CPCC12N1/02C12Q1/18C12Q1/04A01N63/27G01N2333/21Y02A40/10
Inventor 邱睿李小杰苗圃李成军王遂法陈玉国赵均白静科李淑君徐敏何雷何晓冰郑文明邢国珍刘畅
Owner TOBACCO RES INST HENAN ACADEMY OF AGRI SCI