Novel application of Burkholderia polyphaga ws-fj9
A technology of phagocytic Burkholderia, WS-FJ9, applied in the field of microorganisms, can solve the problems of unknown stability of the antibacterial effect of fermentation broth, and achieve excellent UV stability, good biocontrol potential, and significant inhibitory effect Effect
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Embodiment 1W
[0033] Example 1 WS-FJ9 Determination of Antibacterial Spectribue
[0034] Using a tablet confrontation method: use aseptic punch (5mm diameter) in the activated strain nucleus, mushroom, golden housing cyorphosis, and stempot stems to punch the bacteria to access the bacteria to PDA The center of the plate. Take a ring bacteria, inoculate WS-FJ9 at 3cm from the center of the center of the plate, 3-4D, 3-4D at 28 ° C, when the blank control is full of entire petri dishes, measure the width, judgment Antagonistic. Each processes 3 repetitions. The aphrolibia band width is the distance (mm) between the pathogen of the pathogens to the edge of the bacterial moss.
[0035] like figure 1 As shown, the strain WS-FJ9 has different degrees of antagonism for 4 forest trees, with the suppression effect of strain WS-FJ9 on the pyroficide, which can be in the tablet of WS-FJ9 compared to the control group. It is seen that the growth of the femoralius is inhibited, and the fungus near the bact...
Embodiment 2
[0036] Example 2 Antibacterial spectrum measurement of strain WS-FJ9 sterile fermentation filtrate
[0037] 1) Preparation of sterile fermented filtrate
[0038] Activated strain WS-FJ9 on solid LB plates. The strain WS-FJ9 single bacterius was subjected to a 20 ml liquid LB medium with a vaccinated ring, and the culture 24 h was oscillated in the shaker of 28 ° C, 200R / min. The seed liquid was re-transferred to the LB liquid medium in the amount of 1% inoculation, and the fermentation broth was oscillated at 28 ° C, 200R / min shaker 72 h. The supernatant was obtained by centrifugation of the fermentation liquid 1000R / min for 20 min, filtered with 0.22 μm filter, i.e., a sterile fermentation filtrate.
[0039] 2) Antibacterial activity detection of sterile fermented filtrate
[0040] The sterile fermented filtrate was added to the PDA medium according to the ratio of 10%, and then poured into the petri dish after shaking. The pathogenous mushroom was inoculated into the cente...
Embodiment 3
[0042] Effect of 3 Sribin Filtration Filtration Filter Metabolism
[0043] 1, the preparation of the enzyme solution: Picking the plant pathogenic bacteria block in 20 ml of liquid PDA, then adding the sterile fermentation filtrate of strain WS-FJ9 after 25 ° C. After shaking 48h, the pathogenic bacteria is removed, After repeated cleaning, the bacteria is sued and recorded and recorded using a filter paper. The bacteria was moved to a mortar, and the TRIS-HCl extract (0.05 mol / L, pH = 7.8) was added, and the ice bath was grounded to the paste. The abrasive liquid was centrifuged at 4 ° C, 12000 r / min for 15 min, and the supernatant was taken.
[0044] After the treatment of the sterile fermentation filtrate of WS-FJ9 strains, the formaldehyde content in its bacterial lane was significantly higher than that of the control group after treatment of the sterile fermentation filtrate of the WS-FJ9 strain. image 3 A), indicating that the cell membrane of these pathogenic bacteria i...
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