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Preparation method of polymer secondary antibody based on small molecule probe and secondary antibody

A technology of small molecule probes and polymers, applied in the field of medical detection, can solve the problem of low signal intensity, achieve the effects of good solubility, high synthesis yield and purity, and good application prospects

Pending Publication Date: 2022-06-03
宁波砥凡生物科技有限公司
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  • Application Information

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Problems solved by technology

[0005] The embodiment of the present invention provides a method for preparing a polymer secondary antibody based on a small molecule probe and a secondary antibody, aiming to solve the problem of low signal intensity of the antibody prepared in the prior art method

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  • Preparation method of polymer secondary antibody based on small molecule probe and secondary antibody
  • Preparation method of polymer secondary antibody based on small molecule probe and secondary antibody
  • Preparation method of polymer secondary antibody based on small molecule probe and secondary antibody

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Embodiment Construction

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0041] It is to be understood that when used in this specification and the appended claims, the terms "comprising" and "comprising" indicate the presence of the described features, integers, steps, operations, elements and / or components, but do not exclude one or more The presence or addition of a number of other features, integers, steps, operations, elements, components, and / or sets thereof.

[0042] It is also to be understood that...

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Abstract

The invention discloses a preparation method of a polymer secondary antibody based on a small molecule probe and a secondary antibody, in the method, a water-soluble polymer skeleton is modified by using a chemical method, catalase is combined to a polymer through a chemical bond to form a polymer enzyme structure, and after the small molecule probe taking itaconic acid as a parent is combined with the antibody, the polymer secondary antibody is obtained. And mixing and incubating the polymer enzyme and the small molecule-antibody by using a biochemical coupling method to form a polymer enzyme labeled secondary antibody. The preparation method can be used for preparing a polymer secondary antibody for auxiliary diagnosis of solid tumors by immunohistochemistry. The probe matrix is an itaconic acid small molecule, and can realize specific efficient polymerization reaction of an antibody and an enzyme, so that more enzymes are linked to a single-molecule antibody through a coupling structure to form a compound structure with good solubility and small steric hindrance, and the signal intensity can be improved in an immunohistochemical experiment. The preparation method is safe and environment-friendly, is simple and convenient to operate, does not need harsh conditions, and is high in synthesis yield and purity.

Description

technical field [0001] The present invention relates to the technical field of medical detection, in particular to a preparation method and a secondary antibody of a polymer secondary antibody based on a small molecule probe. Background technique [0002] As the global population grows and life expectancy increases, cancer will become more common and the global cancer burden will increase further. The timeliness, comprehensiveness and accuracy of cancer pathological diagnosis are crucial for patients, and play a decisive role in the study of pathogenesis, diagnosis, prognosis, and treatment effectiveness. With the development and progress of medical science and technology, pathological technology has entered a new era, and the application of new technologies and new equipment and products has continuously promoted the development of pathological technology. Especially in recent years, the combination of immunology, molecular biology technology and pathology technology has r...

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Application Information

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IPC IPC(8): G01N33/535G01N33/574G01N33/53
CPCG01N33/535G01N33/574G01N33/5306
Inventor 高津旭
Owner 宁波砥凡生物科技有限公司
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