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Application of cysteamine as electrochemiluminescence co-reactant

A technology of electrochemistry and co-reactants, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, scientific instruments, etc., can solve problems such as volatile, corrosive, toxic, etc., and achieve strong ECL Enhanced, low biotoxic effect

Pending Publication Date: 2022-06-07
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TPrA has several disadvantages: not only is it toxic, corrosive, and volatile, but it needs to be used at high concentrations (usually up to 100mM) to obtain good sensitivity

Method used

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  • Application of cysteamine as electrochemiluminescence co-reactant
  • Application of cysteamine as electrochemiluminescence co-reactant
  • Application of cysteamine as electrochemiluminescence co-reactant

Examples

Experimental program
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Effect test

Embodiment example 1

[0022] To a 30×50 weighing flask, add 3 mL of 0.1M PBS solution with pH=7.4, and then add ruthenium terpyridine solution to make 50 μM Ru(bpy) 3 2+ Then add cysteamine solution to it respectively to prepare a series of solutions to be detected with different concentrations, the final concentrations of cysteamine are 1mM, 2mM, 3mM, 4mM, 5mM, 10mM, 20mM, 30mM, 40mM respectively , and finally, the three-electrode system was used for electrochemiluminescence detection using an electrochemiluminescence workstation, and the linear relationship between ECL luminescence intensity and cysteamine concentration was established. The results are as follows Figure 4 shown. Among them, the detection conditions were that the scanning rate was 100mV / s, the voltage range was 0-2V, and the photomultiplier high voltage was 600V. The detection limit of this method for cysteamine was 0.333mM (S / N=3). In the electrochemiluminescence system of ruthenium terbipyridine / cysteine ​​amine, in the conce...

Embodiment example 2

[0024] To a 30×50 weighing bottle, add 3mL of 0.1M PBS solution with pH=7.4, then add the cysteamine solution to the detection base solution of 30mM cysteamine, and then add the ruthenium terpyridine solution to it to prepare a series of Different concentrations of the solution to be detected, its Ru(bpy) 3 2+ The final concentrations were 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, and 90 μM, respectively. Finally, a three-electrode system was used for electrochemiluminescence detection using an electrochemiluminescence workstation, and the linearity between the ECL luminescence intensity and the concentration of ruthenium terpyridine was established. relationship, the result is Figure 5 shown. Among them, the detection conditions were that the scanning rate was 100mV / s, the voltage range was 0-2V, and the photomultiplier high voltage was 600V. The detection limit of this method for ruthenium terpyridine was 3.33 μM (S / N=3). In the electrochemiluminescence sys...

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Abstract

The invention discloses application of cysteamine as an electrogenerated chemiluminescence co-reactant, and belongs to the technical field of electrogenerated chemiluminescence analysis methods. Cystamine is used as an electrochemiluminescence co-reactant, tris (bipyridine) ruthenium (Ru (bpy) 3Cl2) is used as a luminous body, and the method is used for electrochemiluminescence detection of tris (bipyridine) ruthenium or cystamine. Compared with existing common co-reactants (tri-n-propylamine, TPrA), the co-reactant has the advantages of strong ECL enhancement, low biotoxicity, capability of being used for cell detection in the future and the like. Experimental results show that the application of cysteamine as the terpyridyl ruthenium electrochemiluminescence co-reactant can be used for efficiently detecting the content of terpyridyl ruthenium or cysteamine, and more importantly, within a certain concentration range, the optimal final concentration of cysteamine and terpyridyl ruthenium in a mixed system is given through experiments.

Description

[0001] Technical Field: The present invention belongs to the technical field of electrochemiluminescence analysis methods, and specifically relates to the application of cysteamine as an electrochemiluminescence co-reactant. Background technique [0002] Electrochemiluminescence (ECL) is a kind of luminescence phenomenon that produces some special substances by electrochemical methods, and then these electro-generated substances or between the electro-biomass and other substances further react. It is the product of the combination of chemiluminescence method and electrochemical method. It retains the advantages of high sensitivity, wide linear range, simplicity and simplicity of the chemiluminescence method, and at the same time has the advantages of good reproducibility, easy control, and good stability. ECL technology has been widely used in immunoassay, nucleic acid hybridization assay and determination of other biochemical substances, which not only greatly promotes the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N21/76
CPCG01N27/416G01N21/76
Inventor 鲁理平高亚芳
Owner BEIJING UNIV OF TECH