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Oligopeptide omics identification method based on polypeptide length and application of oligopeptide omics identification method

An identification method and omics technology, applied in informatics, bioinformatics, for analyzing two-dimensional or three-dimensional molecular structures, etc. In order to achieve the effect of improving efficiency and simplicity, improving efficiency and throughput, and reducing burden due to the lack of spectral results

Active Publication Date: 2022-06-07
WUYI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, tools such as Mascot, Maxquant, and Sequest must rely on known protein databases for peptide analysis, and food protein raw materials often cannot provide complete or accurate sequence databases, which greatly increases the difficulty of short peptide analysis
As a peptide analysis tool for de novo sequencing, Peaks can perform peptide analysis without relying on protein databases. However, in practice, it is found that the results of the analysis of the software are too small to meet the analysis needs of food short peptidomics.
At the same time, there are few studies on the identification and application of short peptidomics in related technologies. Therefore, it is urgent to find a perfect short peptidomics identification method

Method used

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  • Oligopeptide omics identification method based on polypeptide length and application of oligopeptide omics identification method
  • Oligopeptide omics identification method based on polypeptide length and application of oligopeptide omics identification method
  • Oligopeptide omics identification method based on polypeptide length and application of oligopeptide omics identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The target sample analyzed in this example is a glutathione standard, and the specific steps for short peptidomics identification based on the length of candidate polypeptide fragments are as follows:

[0099] (1) First, according to the short peptide length range to be determined, the length range of peptide fragments is preset as 2-4, the amino acid residues in the amino acid residue list are 20 common residues, and the preset secondary ion cluster types are a, b and y ion clusters; among them, the amino acid residue sequence numbers are written consecutively from 0 according to the molecular weight of amino acid residues from small to large, that is, each amino acid residue is assigned a unique and continuous sequence number, as shown in Table 1.

[0100] Table 1:

[0101]

[0102]

[0103] Table 1 is a list of common amino acid residues. With reference to the implementation of the present invention, special amino acid residues can be added according to actual ...

Embodiment 2

[0132] The target sample analyzed in this example is a soybean enzymatic hydrolysis product, and the specific steps for short peptidomics identification based on the length of candidate polypeptide fragments are as follows:

[0133] (1) First, the identification range of the peptide fragment length is preset to be 2-6. The amino acid residues in the amino acid residue catalogue are 20 common residues. Residues are assigned a 0-based, unique and consecutive number, as shown in Table 1 of Specific Example 1.

[0134] (2) Obtain the raw data collected in the mass spectrometry of the substances in the soybean enzymatic hydrolysate product to be tested, including the mass spectrum data of the primary parent ion and the secondary product ion, and further obtain the primary mass spectrum and each ion in the secondary mass spectrum. charge information, and perform ion normalization for each of the above ions. Ion normalization processing refers to converting the multiply charged ions...

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Abstract

The invention discloses a polypeptide length-based oligopeptide omics identification method and application thereof. According to the oligopeptide omics identification method based on the polypeptide length disclosed by the invention, all polypeptide fragments within a specified polypeptide length range can be exemplified, theoretical primary parent ions and secondary daughter ions are generated one by one, and are continuously compared with actual ion spectrogram data collected by substances in a sample; and associating the polypeptide meeting the matching requirement with the corresponding substance to finally realize the identification of the polypeptide sequence structure of the substance in the sample. The method can be completely independent of a protein sequence database, the protein enzymolysis product can be directly retrieved and identified without dead corners and omission, meanwhile, the method adopts a completely matched evaluation mode for short peptides, the identification result is high in accuracy and credibility, and the defects of the current proteomics analysis method and tool can be fully overcome. The method is of great significance in improving the polypeptiomics analysis technology level and promoting industry development.

Description

technical field [0001] The invention relates to the field of peptidomics identification, in particular to a short peptidomics identification method based on the length of a polypeptide and its application. Background technique [0002] After the food-derived protein is processed by modern biological enzymolysis technology and turned into food-derived polypeptide, not only the molecular weight becomes smaller, the digestion and absorption characteristics are greatly enhanced, but also the enzymatic hydrolysis products are endowed with a variety of biological activities, such as antioxidant, Anti-aging, improving memory, etc., have received extensive attention from scientific researchers, consumers and food companies from all walks of life. It is worth noting that, because food proteases are mostly crude enzymes and have a wide range of enzyme action sites, the enzymatic hydrolysis products often contain a large number of small molecular short peptides. At the same time, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B15/20G16B30/00G16B50/00
CPCG16B15/20G16B30/00G16B50/00Y02A90/10
Inventor 徐巨才黄峻洪陈雅君严嘉慧梁姚顺陈静刘万顺范丽琪江桂玲
Owner WUYI UNIV