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Superparamagnetic nanoparticles with function of directionally enhancing angiogenesis, exosome and preparation method and application of superparamagnetic nanoparticles and exosome

An angiogenesis and directional enhancement technology, applied in the field of stem cells, can solve the problems of loss of membrane integrity, low centrifugation efficiency, and long time consumption, and achieve the effects of promoting growth and differentiation, strong proliferation ability, and high safety

Pending Publication Date: 2022-06-17
和携科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, the most commonly used methods for extracting exosomes from mesenchymal stem cells are ultracentrifugation and density gradient centrifugation. Density gradient centrifugation is difficult to achieve large-scale production because of its complicated operation and long time consumption; and ultracentrifugation However, the method has defects such as low output, loss of membrane integrity, and low centrifugation efficiency, and it is difficult to achieve the required use effect.

Method used

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  • Superparamagnetic nanoparticles with function of directionally enhancing angiogenesis, exosome and preparation method and application of superparamagnetic nanoparticles and exosome
  • Superparamagnetic nanoparticles with function of directionally enhancing angiogenesis, exosome and preparation method and application of superparamagnetic nanoparticles and exosome
  • Superparamagnetic nanoparticles with function of directionally enhancing angiogenesis, exosome and preparation method and application of superparamagnetic nanoparticles and exosome

Examples

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preparation example Construction

[0040] The present application provides a method for preparing superparamagnetic nanoparticles with directional enhancement of angiogenesis, the method comprising the following steps:

[0041] (1) Under oxygen-free conditions, the nano-iron oxide and nano-nickel oxide are uniformly dispersed in water to form a precursor solution;

[0042] (2) under stirring, the precursor solution obtained in step (1) is heated to 40-50° C., and 1% aqueous NaOH solution is slowly added dropwise to adjust the pH of the solution to 9-10 to form a suspension;

[0043] (3) under anaerobic conditions, adding nano-graphene to the suspension obtained in step (2), and continuously stirring and heating up to 70-80 ° C, and stirring at this temperature for 1-2 h, cooling and separating the precipitate, The superparamagnetic nanoparticles are obtained.

[0044]Wherein, the weight ratio of the nano-iron oxide, the nano-nickel oxide and the nano-graphene is (2-3):(1-2):0.3. The nano iron oxide is magneti...

preparation example 1-9

[0056] Preparation Examples 1-9 respectively provide a preparation method of superparamagnetic nanoparticles with directional enhancement of angiogenesis.

[0057] The difference between the above preparation examples lies in the weight ratio of each component in the superparamagnetic nanoparticles, as shown in Table 1.

[0058] The preparation method of superparamagnetic nanoparticles with directional enhancement of angiogenesis provided by the above preparation example, the specific steps are as follows:

[0059] (1) Under oxygen-free conditions, the magnetic Fe 3 O 4 Nanoparticles and nano-nickel oxide are uniformly dispersed in water to form a precursor solution;

[0060] (2) under stirring, the precursor solution obtained in step (1) was heated to 45° C., and 1% aqueous NaOH solution was slowly added dropwise to adjust the pH of the solution to 10 to form a suspension;

[0061] (3) under anaerobic conditions, add nano-graphene to the suspension obtained in step (2), an...

Embodiment 1-9

[0067] Embodiments 1-9 respectively provide a preparation method of exosomes.

[0068] The difference between the above embodiments is: superparamagnetic nanoparticles, the superparamagnetic nanoparticles provided in Preparation Examples 1-9 are respectively used in Examples 1-9, as shown in Table 2.

[0069] The preparation method of extracting exosomes using superparamagnetic nanoparticles with directional enhancement of angiogenesis provided in the above embodiments is as follows: figure 1 shown, the specific steps are as follows:

[0070] (1) Cell culture: Human umbilical cord mesenchymal stem cells were subcultured in α-MEM complete medium containing 10% FBS and 1% penicillin at a ratio of 1:3, and the third generation of human umbilical cord mesenchymal stem cells were selected. Stem cells, then passage 3 human umbilical cord mesenchymal stem cells were seeded in 100 mL of mesenchymal stem cell serum-free medium containing nicotinamide, N-n-octyl-D-glucosamine, and medi...

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Abstract

The invention relates to the technical field of stem cells, and particularly discloses a superparamagnetic nanoparticle with a function of directionally enhancing angiogenesis, an exosome and a preparation method and application of the superparamagnetic nanoparticle and the exosome. The preparation method of the superparamagnetic nanoparticles with the function of directionally enhancing angiogenesis comprises the following steps: sequentially dissolving nano iron oxide, nano nickel oxide and nano graphene into water; then adjusting the temperature and the pH value; finally, cooling and separating; the superparamagnetic nanoparticles with the function of directionally enhancing angiogenesis are obtained by using the method; a preparation method for extracting exosomes by using the superparamagnetic nanoparticles with the function of directionally enhancing angiogenesis; an exosome obtained by the preparation method; the invention further discloses application of the superparamagnetic nanoparticles and the exosome with the function of directionally enhancing angiogenesis in the aspect of promoting angiogenesis. According to the invention, the high-content exosome can be simply and quickly obtained, so that the generation capability of blood vessels is remarkably enhanced.

Description

technical field [0001] The present application relates to the technical field of stem cells, in particular to a superparamagnetic nanoparticle and exosome with a function of directional enhancement of angiogenesis, and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells are cells that can survive for a long time, reproduce and differentiate themselves, and exist in almost all tissues. In recent years, mesenchymal stem cells are mostly used in transplantation operations. First, mesenchymal stem cells are collected from the autologous body, then isolated and cultured, and finally the proliferated mesenchymal stem cells are reinjected into the body, through the self-targeting of the cells themselves. Sexual function reaches damaged organs and tissues, thereby repairing diseased tissues and cells, and then rebuilding normal tissues and cells. However, autologous mesenchymal stem cells have many defects, such as low in vivo survival...

Claims

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Application Information

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IPC IPC(8): B01J13/02C12N5/0775A61K35/28A61P9/00B82Y30/00
CPCB01J13/02C12N5/0668A61K35/28A61P9/00B82Y30/00C12N2509/10C12N2500/90C12N2500/38C12N2501/91C12N2500/34
Inventor 杨桂花杨海霞赵进军
Owner 和携科技有限公司
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