Method for constructing prokaryote RNA sequencing library

Abstract

The invention provides a method for constructing a prokaryote RNA sequencing library. According to the method, an extension sequence can be added to prokaryote mRNA, and prokaryote RNA can be efficiently called and reversely transcribed by using the extension sequence; meanwhile, according to the method, a molecular tag can be marked for each prokaryote original mRNA molecule, so that the influence of PCR preference on the detection accuracy is avoided, and a prokaryote mRNA sequencing library with low preference and high accuracy is obtained. Compared with a traditional prokaryote RNA sequencing library construction method, the method has the advantages that the constructed library is low in preference, meanwhile, the method is easy and convenient to operate, the success rate is high, and the method is also suitable for detection of samples with extremely low initial quantity (smaller than 2ng total RNA).

Description

technical field

[0001] The invention relates to a method for constructing a prokaryotic RNA sequencing library, which belongs to the technical field of gene sequencing. Background technique

[0002] With the improvement of gene sequencing technology and the successive development of major projects such as the Human Genome Project, the Cancer Genome Project, and the Meta-Hit Project, genome research and RNA sequencing have increasingly become powerful tools for biological and medical research. Numerous new findings in biological and medical research are discovered or validated by RNA sequencing. However, most of the current RNA research objects are eukaryotes, which is also related to the accuracy and stability of the eukaryotic RNA sequencing library construction method is better than the prokaryotic sequencing library construction method. Therefore, the prokaryotic RNA research Better support for RNA sequencing library construction methods is needed.

[0003] The messenge...

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