Uncompressed channel-based unicellular biophysical property detection device and unicellular biophysical property detection method

Abstract

The invention provides a single-cell biophysical property detection device and method based on a non-compression channel. The method comprises the following steps: carrying out fluorescent staining on a cell nucleus of a cell to be detected; injecting the dyed to-be-detected cells into the micro-fluidic chip, and driving the to-be-detected cells to flow in a cell detection channel of the micro-fluidic chip in a non-compressed form by using a negative pressure driving module; the impedance detection module is used for recording direct-current resistance data and multiple pieces of high-frequency impedance data of the to-be-detected cells flowing in the cell detection channel, and the high frequency range is 100 kHz to 1 GHz; recording fluorescence intensity data of the cell nucleus of the cell to be detected flowing in the fluorescence detection window by using a photoelectric detection module; the data analysis and processing module is used for calculating the biophysical characteristics of the to-be-detected cells according to the direct-current resistance data, the multiple pieces of high-frequency impedance data and the fluorescence intensity data, and the biophysical characteristics of the to-be-detected cells comprise the cell diameter, the cell nucleus diameter, the cell membrane specific capacitance and the cytoplasm conductivity.

Description

technical field [0001] The present disclosure belongs to the field of biological detection, and in particular relates to a single-cell biophysical property detection device and method based on an uncompressed channel. Background technique [0002] Cells are the basic structures and units that sustain life. The material and energy intake, transformation, storage and utilization of organisms, and the storage, processing and expression of biological information are inseparable from cells. The study of cells is of great significance for exploring the basic laws of life activities of organisms. The early cell research method was population cell analysis, and the statistical average results obtained could not reflect the biological information differences between single cells. Therefore, the necessity of the importance of single cell analysis in recent years has gradually been reported. [0003] Single-cell analysis usually includes the detection of biochemical properties and th...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the microfluidic impedance cell detectors in the prior art can detect cell size and impedance information, and can achieve higher detection throughput than traditional fixed cell characterization methods, but their detection parameters are less, and Unable to quantify the inherent characteristics of cells, lack of detection versatility

The microfluidic impedance cell detector based on the compression channel has realized the detection of cell diameter, cell membrane specific capacitance, and cytoplasmic conductivity, but the cross-sectional area of ​​the channel used in this method is small, which easily leads to blockage of the cell detection channel, and the detection duration is relatively long. short, and ignores the biophysical properties of the nucleus

[0005] In short, the current single-cell biophysical detection methods have the problems of few detection parameters and easy channel blockage.

Images