Undaria pinnatifida/thermal spring water fermentation liquor, product containing same, and preparation method and application thereof
A technology for hot spring water and wakame, which is applied in the field of fermentation to reduce environmental pollution, reduce production costs, and expand research progress.
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Embodiment 1
[0081] (1) commercial wakame is dried, pulverized, sieved, and the preferred powder particle size is 100 mesh wakame powder; get 300mL of hot spring water (the hot spring water of Conghua hot spring sampling point A, the hot spring water temperature is 36 ℃) and 4.5g of wakame powder was mixed evenly, sterilized in a high-temperature sterilization pot with a temperature of 121°C and a pressure of 0.12MPa for 30min, and cooled to room temperature after sterilization to obtain a fermentation substrate;
[0082] (2) Mix Lactobacillus plantarum plant subsp. (CICC 20261) with hot spring water, and configure to 10 9 CFU / mL of Lactobacillus plantarum liquid; 3 mL of Lactobacillus plantarum liquid was added to the fermentation substrate for fermentation culture; the viable count of Lactobacillus plantarum in the fermentation system was 10 7 CFU / mL (based on the volume of hot spring water in the fermentation substrate), cultured at 43°C for 15h; after the fermentation was completed, s...
Embodiment 2
[0084] Compared with Example 1, the difference is only that the fermentation time is 20h, and other conditions and parameters are the same as those of Example 1.
Embodiment 3
[0086] Compared with Example 1, the difference is only that the bacterial species inoculated in the step (2) is different, and is specially mixed with Lactobacillus plantarum plant subspecies and hot spring water, and is configured into 10 9 CFU / mL of Lactobacillus plantarum plant subsp. bacterial solution; mix Lactobacillus helveticus with hot spring water, and prepare 10 9 CFU / mL of Lactobacillus helveticus bacterial liquid; then take 1.5 mL of Lactobacillus plantarum plant subsp. bacterial liquid with preservation number of CICC 20261 and 1.5 mL of Lactobacillus helveticus bacterial liquid with preservation number of CICC 20243, two kinds of Lactobacillus in the fermentation system The ratio of the inoculum is 1:1, and the total inoculum of the two strains in the fermentation system is 10 7 CFU / mL, other condition parameters are the same as Example 1.
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