Oral spray production process based on gene recombination anti-mutation virus bacteria and fungi

Abstract

The invention relates to the technical field of antiviral oral spray production, and discloses an oral spray production process based on gene recombination anti-mutation virus bacteria and fungi, which comprises the following steps: preparing a virus bacteria antigen through gene recombination; selecting immune animals; carrying out animal immunization, and collecting eggs one week after the last immunization; extracting an egg yolk antibody IgY and a corresponding antibody from the egg yolk; the extracted antibody is detected; and detecting the effect of the antibody on the corresponding type of virus, and preparing the oral spray by using the extracted egg yolk antibody IgY. According to the oral spray production process based on gene recombination anti-mutation virus bacteria and fungi, laying hens are immunized by using S protein of novel coronavirus, a novel coronavirus antibody IgY composite antibody is prepared, the prepared composite antibody not only contains an S1 antibody, but also contains an S2 antibody and the like, and the composite antibody plays a role in various types of viruses and has a good anti-mutation effect on various types of viruses. If the virus is mutated, the spray still has a good prevention and treatment effect.

Description

technical field [0001] The invention relates to the technical field of antiviral oral spray production, in particular to an oral spray production process based on genetic recombination and anti-mutant virus bacteria and fungi. Background technique [0002] The acute severe respiratory syndrome epidemic caused by the new coronavirus infection has spread all over the world since its outbreak. Neutralizing antibodies can neutralize the virus and make the virus lose its infectivity to achieve prevention and control. In COVID-19, the S protein on the surface of the virus mediates receptor recognition and membrane fusion. During virus infection, the trimeric S protein is cleaved into S1 and S2 subunits, and the receptor binding domain (RBD) of the S1 protein directly binds to the peptidase domain (PD) of the target cell surface receptor ACE2, While S2 is responsible for membrane fusion. It can specifically recognize and bind to human cell surface receptors, invade human cells, a...

AI Technical Summary

Problems solved by technology

In addition, due to the lack of plasma sources of recovered patients, it is difficult to meet the treatment needs of a large number of patients.

Many scientists have turned to the development of humanized neutralizing antibody IgG for the new coronavirus, but humanized neutralizing antibody IgG sometimes produces "antibody-dependent enhancement (ADE)" after binding to the virus, assisting the virus to enter the target cell and increasing the infection rate , resulting in an increase of dozens or even hundreds of times in the virus fatality rate. The new coronavirus constantly mutates to form different strains. At present, a single antibody can only deal with a single strain of the new coronavirus, and cannot be universal. Therefore, a new method is proposed Production process of oral spray based on gene recombination anti-mutant new coronavirus

[0004] In addition, for rhinitis caused by viruses or bacterial fungi, antibiotics and antifungal chemical drugs are mainly used in the industry for treatment, but antibiotics generally kill bacteria with a spectrum, which will cause flora imbalance, and antifungal drugs cannot completely cure them; for Helicobacter pylori, asthma , dental caries and other diseases that affect human life, the current treatment methods also have the above disadvantages

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