New application of magnolol

A technology of honokiol and its purpose, applied in the field of medicine, can solve the problems of optic nerve protection that have not been reported, and achieve the effects of reducing the loss of retinal ganglion cells, improving retinal function, and maintaining stable properties.

Pending Publication Date: 2022-07-12
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protective effect of magnolol on the optic nerve in glaucoma disease characterized by high intraocular pressure has not been reported so far.

Method used

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  • New application of magnolol
  • New application of magnolol
  • New application of magnolol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of mouse intraocular hypertension model and honokiol intervention

[0048] 1. Establishment of mouse intraocular hypertension model:

[0049] All experiments were carried out under general anesthesia in mice, and the mice were purchased from Shanghai Jisijie Laboratory Animal Co., Ltd. 6-8 week old female C57BL / 6 mice were selected, anesthetized with zolitinib / dexmedetomidine mixture, and the right eye underwent high intraocular pressure injury. A 30-gauge needle was inserted into the anterior chamber. The needle was sterile with 100ml The isotonic saline bag was connected, the height of the saline bag was 120 cm from the mouse eyeball, and the injury was induced for 60 minutes. The intraocular pressure was measured at about 90 mmHg at this time. Mice without intraocular hypertension injury were used as a control group.

[0050] 2. Honokiol intervention:

[0051] Mice were injected intraperitoneally with honokiol (25 mg / kg, Selleckchem, Housto...

Embodiment 2

[0052] Example 2: Honokiol can improve the survival rate of retinal ganglion cells

[0053] 1. Assessment of optic nerve injury

[0054] Optic nerve damage was detected 7 days after IOP injury. The mouse hearts were perfused with normal saline for 5 minutes to remove blood cells, and then injected with 4% paraformaldehyde for 15 minutes for in situ fixation, and then the eyeballs were removed. Eyeballs were fixed with 4% PFA for 2 hours. Retinas were dissected to a tiled state, fixed with 4% PFA for 15 min, and permeabilized with 0.3% triton x-100 in PBS for 15 min. Retinas were blocked with 5% goat serum (Boster, CA, USA)-0.3% triton x-100 in PBS for 2 hours at room temperature, and then with β-III tubulin (a marker for RGCs; 1:1000; Abcam) 4°C overnight, followed by Alexa Fluor 594 anti-mouse secondary antibody (1:200; Abbkine, CA, USA). Four images were taken in each retinal intermediate region by confocal microscopy, and the immunopositive cells were counted with Image...

Embodiment 3

[0057] Example 3: Honokiol can inhibit retinal tissue thinning

[0058] 1. Histomorphological analysis

[0059] Mouse eyeballs were removed 1 and 7 days after ocular hypertension, and the eyeballs were fixed with 4% PFA overnight. Retinal thickness was assessed by morphological analysis of haematoxylin and eosin (H&E) stained retinal frozen or paraffin sections. Retinal nerve fiber layer (RNFL) and inner plexiform layer (IPL) thicknesses were measured on H&E images at the same distance from the optic nerve head and expressed as mean values.

[0060] 2. Analysis results

[0061] like figure 1 As shown in C-D, the thicknesses of retinal nerve fiber layer (RNFL) and inner plexiform layer (IPL) in the AOH1d group did not change significantly compared with the CON group, while the RNFL and IPL in the AOH7d group were significantly thinner compared with the CON group, while AOH7d The RNFL and IPL in the +MAG group increased in thickness compared with the AOH7d group. The experi...

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Abstract

The invention provides a novel application of magnolol. The magnolol disclosed by the invention can be used for reducing retinal ganglion cell loss and retinal damage caused by intraocular hypertension; the visual function reduction caused by intraocular hypertension is improved; the generation of inflammatory factors in retinal tissues caused by intraocular hypertension is inhibited; the activation of microglial cells and astrocytes in the retina caused by intraocular hypertension can be reduced, phenotypic polarization of the microglial cells to M1 can be reduced, and phenotypic polarization of the microglial cells to M2 can be promoted; the decrease of blood retina barrier related protein in the retina tissue caused by intraocular hypertension is relieved; and the increase of the proportion of Th1, Th2 and Th17 cells in ocular reflux lymph nodes caused by intraocular hypertension is reduced. Magnolol can be used for preventing, treating or relieving optic nerve injury caused by glaucoma diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new use of magnolol. Background technique [0002] Glaucoma is the world's largest irreversible blinding eye disease, and it is estimated that by 2040, the number of glaucoma patients will reach 112 million. Glaucoma can cause optic nerve damage and visual field defect, endanger people's health, reduce the quality of life, and affect social and economic development. High intraocular pressure is considered a major risk factor for glaucoma, which results in the loss of retinal ganglion cells. However, the strategy of reducing intraocular pressure has little effect in a considerable number of glaucoma patients. In recent years, neuroinflammatory damage caused by high intraocular pressure has been widely concerned. [0003] Magnolol is a small molecular substance extracted from the traditional Chinese medicine Magnolia officinalis, with a bisphenol structure. It has been report...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P27/06A61P27/02A61P9/10A23L33/10
CPCA61K31/05A61P27/06A61P27/02A61P9/10A23L33/10A23V2002/00A23V2200/30
Inventor 郭文毅孙浩王宁
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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