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Quantitative spectrum chip for calibrating metabolome quantitative analysis

A metabolome and composition technology, applied in the field of accurate quantitative research in metabolomics, to achieve accurate quantitative results, important application value, and the effect of improving reliability

Pending Publication Date: 2022-07-12
常州中科脂典生物技术有限责任公司 +1
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Problems solved by technology

At present, the biggest challenge of non-targeted plasma metabolome research based on LC-MS technology is how to achieve accurate quantification of data to meet the data calibration of different types of complex components

Method used

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  • Quantitative spectrum chip for calibrating metabolome quantitative analysis
  • Quantitative spectrum chip for calibrating metabolome quantitative analysis
  • Quantitative spectrum chip for calibrating metabolome quantitative analysis

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Embodiment 2

[0050] Various life activities in cells, such as signal release and conduction, energy transfer, etc., are reflected at the metabolic level, responding to various endogenous and exogenous stimuli. Metabolomics methods based on LC-MS technology have been widely used in research in many disciplines. However, one of the main challenges of untargeted metabolomics studies is the calibration of mass spectrometry data. In the embodiment of the present invention, a series of isotopic internal standards whose types and contents match with the plasma endogenous metabolites are introduced to calibrate the plasma metabolome data obtained based on non-targeted LC-MS technology. The method calibration data of the matching of species type, the proximity of retention time, and the matching of mass spectral signal intensity, and the selection of calibration internal standard is judged according to the result of the reduction of RSD value of QC after calibration.

[0051] The selection of plas...

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Abstract

The invention discloses a quantity spectrum chip for calibrating quantitative analysis of metabolome, the chip comprises a mixed isotope internal standard composition for calibrating non-targeted plasma metabolome data, and the composition is composed of 64 substances. According to the invention, different proportions of the mixed isotope internal standard are researched to calibrate the blood plasma endogenous metabolite spectrum data; it is found that the combination rule of the minimum RSD value can be obtained by flexibly matching the endogenous metabolite in the plasma with the isotope internal standard of the endogenous metabolite, the isotope internal standard of the same type, the isotope internal standard close to the retention time and the isotope internal standard close to the mass spectrum signal intensity.

Description

technical field [0001] The invention belongs to the field of precise quantitative research in metabolomics, relates to the application of an isotope internal standard group in metabolomics research, and in particular relates to the application of an isotopic internal standard group of representative metabolites in plasma in liquid chromatography-mass spectrometry (LC-MS) technology ) application of precise quantitative analysis in the detection of plasma metabolomes. Background technique [0002] As a powerful tool for qualitative and quantitative research of small molecule metabolites in biological samples, metabolomics has become the main method to annotate gene functions and reveal various endogenous physiological and biochemical reactions of cells after exogenous stimulation, and is widely used. In various research fields, including life sciences, disease diagnosis, drug development, nutrition, toxicology, environmental science, botany and other fields. LC-MS is the mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72G01N30/86
CPCG01N30/06G01N30/7233G01N30/8665G01N2030/045G01N2030/027G01N2030/8822
Inventor 税光厚田鹤林茜雯
Owner 常州中科脂典生物技术有限责任公司
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