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Modified bacterial cellulose beneficial to cell culture and application thereof

A technology of bacterial cellulose and cell culture, applied in tissue culture, microorganisms, biochemical equipment and methods, etc., can solve the problem that bacterial cellulose scaffolds cannot be directly applied to cell culture meat, etc., and achieve good cell adhesion and proliferation, preparation method Simple, Inexpensive Effects

Pending Publication Date: 2022-07-15
上海食未生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, these bacterial cellulose scaffolds cannot be directly applied in cell culture meat

Method used

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  • Modified bacterial cellulose beneficial to cell culture and application thereof
  • Modified bacterial cellulose beneficial to cell culture and application thereof
  • Modified bacterial cellulose beneficial to cell culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Bacterial cellulose / chitosan composite porous scaffold

[0049] (1) Cultivate microorganisms to generate bacterial cellulose and purify it. Acetobacter xylinum Komagataeibacter xylinus Bacterial cellulose was obtained by superficial static culture at 30°C for 14 days. It was taken out, washed with deionized water to remove most of the residual liquid, then boiled in 0.1 M NaOH solution for 30 min to remove cells and pyrogens, and then repeatedly soaked and washed with deionized water until the pH became neutral.

[0050] (2) Physical method to disperse bacterial cellulose. The bacterial cellulose was initially crushed with a rotating blade for 3 min, then sheared at a high speed of 15,000 rpm for 5 min, and then crushed at 720 W ultrasonic intensity for 5 min to obtain a bacterial cellulose dispersion.

[0051] (3) Preparation of scaffold dispersion. First, 2 wt% chitosan in 1 wt% acetic acid aqueous solution was prepared. Then, according to the dry weig...

Embodiment 2

[0055] Example 2 Bacterial cellulose / polylysine composite porous scaffold

[0056] (1) Cultivate microorganisms to generate bacterial cellulose and purify it. Acetobacter xylinum Komagataeibacter xylinus Bacterial cellulose was obtained by shaking at 100 rpm for 7 days at 28 °C. It was taken out, washed with deionized water to remove most of the residual liquid, then boiled in 0.1 M NaOH solution for 30 min to remove cells and pyrogens, and then repeatedly soaked and washed with deionized water until the pH became neutral.

[0057] (2) Physical method to disperse bacterial cellulose. The bacterial cellulose was initially crushed with a rotating blade for 3 min, then crushed at 720 W ultrasonic intensity for 5 min, and then subjected to high-speed shearing at 15,000 rpm for 5 min to obtain a bacterial cellulose dispersion.

[0058] (3) Preparation of scaffold dispersion. First prepare a 2 wt% polylysine aqueous solution. Then, according to the dry weight ratio of bacteria...

Embodiment 3

[0062] Example 3 Bacterial cellulose directional scaffold

[0063] (1) Cultivate microorganisms to generate bacterial cellulose and purify it. Acetobacter xylinum Komagataeibacter xylinus Bacterial cellulose was obtained by shaking at 150 rpm for 10 days at 26 °C. It was taken out, washed with deionized water to remove most of the residual liquid, then boiled in 0.1 M NaOH solution for 30 min to remove cells and pyrogens, and then repeatedly soaked and washed with deionized water until the pH became neutral.

[0064] (2) Physical method to disperse bacterial cellulose. The bacterial cellulose was initially crushed with a rotating blade for 3 min, then sheared at a high speed at 18,000 rpm for 5 min, and then crushed at 600 W ultrasonic intensity for 5 min to obtain a bacterial cellulose dispersion.

[0065] (3) Preparation of oriented porous scaffolds. The bacterial cellulose dispersion was poured into one of the two hollow areas of the directional freezer and covered wit...

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Abstract

The invention belongs to the field of biology, and discloses modified bacterial cellulose beneficial to cell culture and application of the modified bacterial cellulose. The modified bacterial cellulose is obtained by dispersing purified bacterial cellulose and a modifier in a solvent and fully reacting. The modified bacterial cellulose can be used for preparing a scaffold beneficial to cell culture. When the modified bacterial cellulose of some examples is used for cell culture meat, cell adhesion growth can be well promoted, and meanwhile the produced cell culture meat has good taste; the preparation method is simple, efficient, high in reproducibility and low in cost. Scaffolds of some embodiments of the present invention do not contain animal components, do not require the addition of ECM proteins, and exhibit good cell adhesion and proliferation at the same time. The scaffold is edible, and chemical cross-linking agents and toxic and harmful solvents are not added. The scaffold does not need to be separated or degraded, can be directly used as a component of a cell culture meat final product, and is favorable for simplifying a culture meat production process and reducing the manufacturing cost.

Description

technical field [0001] The invention belongs to the biological field, and in particular relates to a modified bacterial cellulose beneficial to cell culture and its application. Background technique [0002] Cell cultured meat is a meat production method that uses a small amount of animal cells to culture, and obtains muscle, fat and other tissues through cell growth, proliferation, and differentiation. Compared with traditional meat production methods, it has higher energy conversion rate, less dependence and damage on the environment, clean and hygienic production process, no added hormones and antibiotics, and less harm to animals. , Green and sustainable meat products respond to supply pressures and fill demand gaps. [0003] The four key technologies in the field of cell culture meat are: (1) isolation and culture of muscle and fat cells; (2) preparation of xeno-free medium; (3) bioreactor design; (4) scaffold development. The scaffold has a rich pore structure, which...

Claims

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Application Information

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IPC IPC(8): C08G81/00C08B15/06C12N5/00
CPCC08G81/00C08B15/06C12N5/0068C12N2533/78C12N2533/72C12N2533/32
Inventor 张玺铭唐雨楠向宁
Owner 上海食未生物科技有限公司
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