Primer, probe and kit for fluorescent quantitative PCR (polymerase chain reaction) detection of pigeon rotavirus A

Abstract

The invention provides a primer, a probe and a kit for fluorescent quantitative PCR (Polymerase Chain Reaction) detection of pigeon rotavirus type A. The primer sequence is as follows: a primer PiRVA-TF: 5 '-CCGAACTGCTCCTGTATGAAT-3', a primer PiRVA-TR: 5 '-CATTGCCATTGCTATCCATT-3', a primer PiRVA-TR: 5 '- The probe is a probe PiRVA-probe: 5 '-AGTCTGACATCATTTCATTGCACAACC-3', the 5 '-end of the probe is marked with a fluorescence report group FAM, the middle and the 3'-end of the probe are respectively marked with a quenching group DBQ1, the method established by the invention is high in sensitivity, good in stability, strong in specificity and good in repeatability, can be used for specific detection of the pigeon rotavirus type A, and can be applied to detection of the pigeon rotavirus type A. And a foundation is laid for the subsequent scientific research on the pathogenic mechanism of the pigeon rotavirus A and the development of molecular epidemiology.

Description

technical field [0001] The invention belongs to the field of animal virology, and in particular relates to a primer and a probe for the detection of pigeon rotavirus type A fluorescence quantitative PCR and a kit thereof. Background technique [0002] Humans have a long history of raising pigeons. Pigeons have been domesticated into ornamental pigeons, racing pigeons and meat pigeons. They have a strong immune system. In addition, the lofts are mostly open or semi-open, with good air circulation and fresh air. Poultry and pigeons are less sick. However, with the rapid development of large-scale and intensive pigeon breeding industry, the increase of the total amount and the stocking density, the way of raising pigeons has changed. Frequent (including homing pigeon races), pigeon diseases are becoming more and more serious and more complicated. In recent years, pigeon viral infectious diseases have seriously threatened the pigeon industry. According to domestic and foreign ...

AI Technical Summary

Problems solved by technology

Rotavirus type A (Rotavirus A, RVA) generally infects animals of a specific population, and such viruses are called homologous strains, but occasionally cross-species transmission occurs

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