Aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application thereof

Abstract

The invention relates to an aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application, the kit comprises an immunochromatography test strip and an aggregation-induced emission fluorescent probe, the immunochromatography test strip comprises a polyvinyl chloride base plate, the upper surface of the polyvinyl chloride base plate is provided with a nitrocellulose membrane, a water absorption pad and a sample pad, and the nitrocellulose membrane is arranged on the upper surface of the polyvinyl chloride base plate. A detection line T1, a detection line T2 and a quality control line C are sequentially arranged on one side, close to the sample pad, of the upper surface of the nitrocellulose membrane; the aggregation-induced emission fluorescent probe comprises a green aggregation-induced emission fluorescent microsphere probe prepared from BTPETD and a red aggregation-induced emission fluorescent microsphere probe prepared from TPB. The device has the advantages of convenience in use and simplicity in operation. The method is used for qualitative and quantitative detection of the furaltadone metabolite and the nitrofurantoin metabolite at the same time, the detection efficiency is greatly improved, and the detection sensitivity can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic detection, relates to an aggregation-induced luminescence immunochromatography kit for detecting two nitrofuran metabolites and its application, in particular to a fluorescent immune layer prepared by using aggregation-induced luminescence fluorescent microspheres as a labeled carrier Analytical test strips for simultaneous detection of nitrofurantoin metabolites (AMOZ) and nitrofurantoin metabolites (AHD) in aquatic products. Background technique [0002] Nitrofurans have been widely used in livestock and poultry and aquaculture because of their low price and good effect, for the treatment of gastrointestinal infections and the prevention of microbial infections caused by lesions. Because nitrofurans and their metabolites have side effects such as carcinogenicity and mutagenicity to humans, many countries, including my country, have long banned their use in animal food production. Nitrofuran ...

AI Technical Summary

Problems solved by technology

CN113156112A discloses a fluorescent immunochromatography kit for simultaneously detecting four nitrofuran metabolites and its preparation method and application. The method uses quantum dot fluorescent microsphere-labeled antibodies to detect nitrofuran drug metabolites Fluorescent immunochromatographic test strips, the markers used in the kit will undergo fluorescence quenching in the case of aggregation, that is, the aggregation-induced quenching effect. When the concentration of the target to be detected is low, this aggregation-induced quenching The labeling method of fluorescent materials still limits the detection of immunochromatographic test strips, which affects the detection accuracy and sensitivity

Images