Sample treatment method for ruminant rumen microorganism research

Abstract

The invention relates to a sample treatment method for ruminant rumen microorganism research, which comprises a sample selection method and a sample treatment method and process, the sample selection mode is divided into four modes, and the four modes are respectively a solid-liquid mixed sample, a solid-liquid simultaneous selection, only a solid phase sample and only a liquid phase sample. The sample treatment method and process comprise three steps of sampling time selection, sample collection and sample treatment, and the sample treatment comprises liquid phase sample treatment and solid phase sample treatment. The sample treatment method for ruminant rumen microorganism research has the beneficial effects that rumen microorganism research is carried out by adopting a solid-liquid separation method, deviation generated by selecting rumen fluid for research can be effectively avoided, meanwhile, target species can be more comprehensively screened out, and the sample treatment method is simple and convenient to operate. And the problem of hybrid genome pollution generated when a metagenome technology is used for research can be improved to a great extent.

Description

technical field [0001] The invention relates to the technical field of ruminant rumen microorganism research, in particular to a sample processing method for ruminant rumen microorganism research. Background technique [0002] Ruminants are distinguished from other animals by their unique rumen. The rumen is a complex and dynamic ecosystem mainly composed of microorganisms such as anaerobic bacteria, anaerobic fungi, protozoa, methanogenic archaea, and bacteriophages. Microbes interact closely with each other and symbiotic with the host by providing nutrients such as microbial protein (MCP), volatile fatty acids (VFA), and vitamins to the parasitic host. A healthy rumen is the foundation for ruminant health, welfare and maintaining high productivity. [0003] The current mainstream rumen microbiome research model has certain flaws. It is mainly manifested in the following aspects: 1. Selecting rumen juice for research may lead to biased results. Because some studies based...

AI Technical Summary

Problems solved by technology

Therefore studies using rumen fluid may not be comprehensive

2 Although the selection of rumen chyme avoids the above-mentioned problems, the current sequencing technology may cover some species that are not high in abundance but play an important role

But if you use raw chyme, it may be overwritten

3. Regardless of whether it is rumen fluid or rumen digesta samples, when conducting metagenomic sequencing technology research, they will be polluted by genomes from feed plants (the pollution of the host itself is relatively simple and can be eliminated, but the composition of the feed is relatively complex and completely almost impossible to remove)