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Construction method of model of diarrhea of young rabbits infected by vibrio parahaemolyticus

A technology of Vibrio hemolyticus and construction method, which is applied in the direction of resisting vector-borne diseases, animal husbandry, etc., can solve the problems of few operations, short modeling experiment time, and high order of magnitude infection with Vibrio parahaemolyticus, and the number of operations can be reached. Less time, short modeling experiment time, obvious effect of clinical symptoms and pathological changes

Pending Publication Date: 2022-08-05
SHANGHAI OCEAN UNIV
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  • Description
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Problems solved by technology

[0005] In order to overcome the problems of poor controllability, instability, low success rate and difficulty in establishing a general laboratory in establishing an animal model of Vibrio parahaemolyticus infection in the prior art, the object of the present invention is to provide a vibrio parahaemolyticus-infected animal model. The construction method of the rabbit diarrhea model, the modeling experiment time is short, the number of operations is small, the order of infection of Vibrio parahaemolyticus is high, and the application range is wide

Method used

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  • Construction method of model of diarrhea of young rabbits infected by vibrio parahaemolyticus
  • Construction method of model of diarrhea of young rabbits infected by vibrio parahaemolyticus
  • Construction method of model of diarrhea of young rabbits infected by vibrio parahaemolyticus

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Embodiment 1

[0028] The environment in the following examples are all carried out in an SPF environment, specifically: the temperature is 30 ± 2 °C, the humidity is 65 ± 5%, the bedding material is replaced every day, the light is controlled for 12 hours, and the darkness is controlled for 12 hours; feed: small pet formula sheep Milk powder; eat every 3–4 hours. Dissections were performed in a ventilated ultra-clean bench.

[0029] 1. Infection mode

[0030] Infection by gavage. By intraperitoneal injection of New Zealand baby rabbit cimetidine injection (50mg / kg) 3h, with 2.5% sterile NaHCO 3 The solution is to resuspend the Vibrio parahaemolyticus bacterial solution to adjust its final concentration to the concentration required for infection, and use a flexible gavage needle to gavage the bacterial suspension of young rabbits.

[0031] 2. Evaluation indicators

[0032] To better assess the diarrhea status of the young rabbits, diarrhea status was recorded every 3 hours, and diarrhea...

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Abstract

The invention discloses a construction method of a vibrio parahaemolyticus infected young rabbit diarrhea model, and relates to a food-borne pathogenic bacterium infection model. The construction method comprises the following steps: taking a two-day-old New Zealand young rabbit as a test animal, fasting the obtained young rabbit, performing intragastric administration with vibrio parahaemolyticus, feeding after 8-12 hours, observing the inoculated young rabbit and recording the death condition, dissecting and sampling the dead young rabbit, and recording obvious diarrhea symptoms. The construction method disclosed by the invention is short in experimental time, few in operation times and high in order of magnitude of infected vibrio parahaemolyticus, clinical symptoms and pathological changes of an infected animal model can correctly reflect the diarrheogenic virulence of the vibrio parahaemolyticus, and the model animal is small in size, easy to operate and suitable for being used as a research model of the vibrio parahaemolyticus.

Description

technical field [0001] The invention relates to a food-borne pathogenic bacteria infection model, which is a method for constructing a diarrhea model of a young rabbit infected with Vibrio parahaemolyticus, belonging to the field of biomedicine, in particular to the field of pathology. Background technique [0002] Vibrio parahaemolyticus is an important food-borne pathogen. People often suffer from acute gastrointestinal symptoms with abdominal pain, diarrhea, nausea, vomiting and fever as the main symptoms after eating food contaminated by Vibrio parahaemolyticus. inflammation. One of its characteristics is that the stool of the poisoned person is mostly watery, and in severe cases, dehydration, shock coma, and even death may occur. In recent years, the pathogenicity of Vibrio parahaemolyticus has far surpassed that of Salmonella, ranking first among food-borne pathogens. In view of its extremely strong infection rate and serious morbidity, it has received close attention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/25A01K2227/107A01K2267/0337Y02A50/30
Inventor 赵勇徐欢惠潇然张昭寰刘静
Owner SHANGHAI OCEAN UNIV
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