Silkworm chrysalis protein powder and preparation method thereof
A silkworm chrysalis protein powder and silkworm chrysalis technology are applied in the field of silkworm chrysalis protein powder and its preparation, and can solve problems such as unpalatable flavor, and achieve the effects of enhancing flavor components and enhancing palatability.
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[0023] One embodiment of this application provides a preparation method of silkworm pupa protein powder, including:
[0024] Dry the dry silkworm pupa and crushed it for sub -treatment, and get the material one;
[0025] Once the material is soaked in alkaline, centrifugation, collecting precipitation to get material two;
[0026] Drink the material of the material to get the material three;
[0027] Material three is used for Marada to get silkworm pupa protein.
[0028] In this embodiment, the dehydration treatment is: mixing the ratio of dry silkworm pupa and petroleum ether according to the proportion of the material to 1: 4, the constant warm water bath is abandoned for 4h under 50 ° C, and after three times, 3000 R / min is 5min. Remove the liquid liquid, and then wash and dry at 60 ° C to get the materials after skipping.
[0029] In some specific embodiments, the drying temperature is 60 ° C.
[0030] In some specific embodiments, the alkali immersion is added to the materia...
Embodiment 1
[0038] Example 1 Preparation of silkworm pupain powder
[0039] (1) After drying and crushing the dry silkworm pupa at 60 ° C, take 10g of crushed silkworm pupa, add 40ml petroleum ether, place 4h at 50 ° C constant temperature, repeat the fat three times, 3000 R / min centrifuged 5min, remove the cleaner, and then then remove the liquid, then Dry at 60 ° C and get material one;
[0040] (2) Add NAOH solution to the proportion of NAOH solution with a mass score of 1%in 1G material one, add NaOH solution, stir and extract 180min at 55 ° C, centrifugation for 10min under 4000r / min, then adjust the pH to 4.5, and then adjust to 4.5. Take the precipitation and get the material two;
[0041] (3) Add alkalinase and papaya protease to the material two. Among them, the proportion of alkaline protease and papaya protease is 4: 1 weight ratio, enzyme activity is 6000 U / g, and the amount of enzymes is 3% of the material dual weight. The enzymatic disintegration temperature is 55 ° C, and the e...
Embodiment 2
[0043] Example 2 Preparation of Silkworm Big Bun Protein Powder
[0044] (1) After drying and crushing the dry silkworm pupa at 60 ° C, take 10g of crushed silkworm pupa, add 40ml petroleum ether, place 4h at 50 ° C constant temperature, repeat the fat three times, 3000 R / min centrifuged 5min, remove the cleaner, and then then remove the liquid, then Dry at 60 ° C and get material one;
[0045] (2) Add NAOH solution to the proportion of NAOH solution with a mass score of 1%in 1G material one, add NaOH solution, stir and extract 180min at 55 ° C, centrifugation for 10min under 4000r / min, then adjust the pH to 4.5, and then adjust to 4.5. Take the precipitation and get the material two;
[0046] (3) Add alkalinase and papaya protease to the material two. Among them, the proportion of alkaline protease and papaya protease is 4: 1 weight ratio, enzyme activity is 6000 U / g, and the amount of enzymes is 3% of the material dual weight. The enzymatic disintegration temperature is 60 ° C, ...
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