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Fermented production method of floral-scented low-salt fermented bean curd paste

A production method and salt rot technology, which is applied in the field of bioengineering fermentation, can solve the problems of single flavor and high salt content of the product, and achieve the effect of improving flavor components, high dietary fiber content, and fine and uniform texture

Pending Publication Date: 2020-11-24
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems of high salt content in traditional fermented bean curd, potential safety risks after salt reduction, and single flavor of the product, the present invention provides a fermented production method of flower-flavored low-salt fermented bean curd cream and its new product, which can make the salt of the fermented bean curd cream product The concentration is controlled at 3% to 5%. The pre-fermentation and post-fermentation are each inoculated with different functional strains. The fermentation is sufficient, which realizes the low-salinization of the product, eliminates safety risks, and effectively improves the flavor components, functional components and nutritional quality of fermented bean curd.

Method used

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  • Fermented production method of floral-scented low-salt fermented bean curd paste
  • Fermented production method of floral-scented low-salt fermented bean curd paste
  • Fermented production method of floral-scented low-salt fermented bean curd paste

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Embodiment 1

[0029] The preparation method of embodiment 1 low-salt fermented bean curd, comprises the steps:

[0030] (1) Domestication and improvement of Mucor radiata and Aspergillus oryzae

[0031] Add 0-0.04% concentration of L-tyrosine to the PDA agar medium, inoculate Mucor actinosa and Aspergillus oryzae, gradually increase the concentration of L-tyrosine, and carry out domestication by repeated passage for 15 generations. The activity of phthaloyl tyrosine hydrolase in Mucor actilii and Aspergillus oryzae decreased significantly, and their ability to produce tyrosine decreased by 34.3% and 32.5% respectively compared with unaccustomed strains, and the white spot rate of sufu decreased.

[0032] (2) Preparation of starter

[0033] Inoculate Monascus, domesticated Mucorus elegans, and domesticated Aspergillus oryzae on PDA plate medium, culture at 28°C for 3-5 days, elute with 0.8% sterile water, and homogenize at 1500r / min for 5 minutes with a high-speed homogenizer to prepare Th...

Embodiment 2

[0046] Example 2 Antibacterial activity of bacteriocin produced by Enterococcus faecalis and its application in fermented bean curd paste fermentation

[0047] Enterococcus faecalis (E.Faecium) CGMCC No.16677 used in the present invention and the biological characteristics, functional identification, and antibacterial effect of the bacteriocin produced by it can be found in the patent "A bacteriocin Gr17 with broad-spectrum antibacterial activity and its application "(public number: CN109627299A). The Enterococcus faecalis Gr17 was isolated from traditional Chinese low-salt fermented whole fish products, and its complete genome sequence was determined; The molecular weight of coccus bacteriocin Gr17 is 4531.01Da; bacteriocin Gr17 is effective against Listeria monocytogenes (Listeriamonocytogenes), Staphylococcus aereus (Staphylococcus aereu), Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli), Sakazaki intestinalis Enterobacter sakazakii, Enterococcus ...

Embodiment 3

[0051] Example 3 The active ingredient of Monascus CGMCC No.12502 and its application in fermented bean curd cream

[0052] For the biological characteristics and the active ingredients produced by the Monascus CGMCC No.12502 used in the present invention, see the patent "a blood fat-lowering oatmeal red yeast tea and its preparation method" (publication number: CN106173054A). The bacterium is the "M1" strain selected by the applicant's team through mutagenesis, and the preservation number is "CGMCC No. 12502". The content of Monacolin K in the fermented liquid of M1 strain detected by HPLC reached 421.7mg / L, which was 142.1% higher than that of the starting strain M1, and the performance of producing Monacolin K after 10 subcultures only decreased by 4.3%, and the genetic stability was good, indicating that M1 The strain has the characteristics of high production of functional component Monacolin K. The breeding process and characteristics of the M1 strain are detailed in th...

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Abstract

Fermented bean curds are a traditional fermented spice in China, and mainly characterized by high salt content. In recent years, low-salt diets beneficial to the health have been widely recognized bythe public. Traditional fermented bean curd products are basically blocky and mainly suitable for daily diets. In recent years, dipping sauce for hot pot and baked foods require dilute fermented beancurd spice. The invention provides a fermented production method of floral-scented low-salt fermented bean curd paste. By coupling treatment and synergistic effect of a variety of functional bacteriaand compound enzyme preparations, the formation of flavor substances, taste substances and functional components of the fermented bean curd paste is promoted, the potential safety risk is eliminated and low salt content of the fermented bean curd paste product is achieved. The fermented bean curd paste is moderate in saltiness, palatable, fine in texture, prominent in mellow fragrance and floral scent, high in dietary fiber content, and capable of being used as a directly smeared spice.

Description

technical field [0001] The invention relates to a fermentation production method and application of flower-flavored low-salt fermented bean curd paste, belonging to the technical field of bioengineering fermentation. Background technique [0002] Fermented bean curd, also known as fermented bean curd, moldy tofu, and sauced tofu, is a traditional fermented soybean food in my country. Sufu contains various nutrients such as free amino acids, peptides, calcium, phosphorus, riboflavin and B vitamins, and contains functional ingredients such as antioxidant peptides, ACE inhibitory peptides, soybean isoflavones, etc. Physiological activity such as tumors. [0003] At present, the main method of fermented bean curd production is to inoculate a single pure-bred microorganism for pre-fermentation to make the dough, and the post-fermentation process belongs to natural fermentation. According to the microorganisms inoculated before fermentation, fermented bean curd can be divided in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C20/02A23L11/30
CPCA23C20/025A23L11/37A23L11/33A23L11/30
Inventor 王成涛刘国荣赵磊张婵郝帅程雷孙宝国王欣赵丹
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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