Fermented production method of floral-scented low-salt fermented bean curd paste
A production method and salt rot technology, which is applied in the field of bioengineering fermentation, can solve the problems of single flavor and high salt content of the product, and achieve the effect of improving flavor components, high dietary fiber content, and fine and uniform texture
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] The preparation method of embodiment 1 low-salt fermented bean curd, comprises the steps:
[0030] (1) Domestication and improvement of Mucor radiata and Aspergillus oryzae
[0031] Add 0-0.04% concentration of L-tyrosine to the PDA agar medium, inoculate Mucor actinosa and Aspergillus oryzae, gradually increase the concentration of L-tyrosine, and carry out domestication by repeated passage for 15 generations. The activity of phthaloyl tyrosine hydrolase in Mucor actilii and Aspergillus oryzae decreased significantly, and their ability to produce tyrosine decreased by 34.3% and 32.5% respectively compared with unaccustomed strains, and the white spot rate of sufu decreased.
[0032] (2) Preparation of starter
[0033] Inoculate Monascus, domesticated Mucorus elegans, and domesticated Aspergillus oryzae on PDA plate medium, culture at 28°C for 3-5 days, elute with 0.8% sterile water, and homogenize at 1500r / min for 5 minutes with a high-speed homogenizer to prepare Th...
Embodiment 2
[0046] Example 2 Antibacterial activity of bacteriocin produced by Enterococcus faecalis and its application in fermented bean curd paste fermentation
[0047] Enterococcus faecalis (E.Faecium) CGMCC No.16677 used in the present invention and the biological characteristics, functional identification, and antibacterial effect of the bacteriocin produced by it can be found in the patent "A bacteriocin Gr17 with broad-spectrum antibacterial activity and its application "(public number: CN109627299A). The Enterococcus faecalis Gr17 was isolated from traditional Chinese low-salt fermented whole fish products, and its complete genome sequence was determined; The molecular weight of coccus bacteriocin Gr17 is 4531.01Da; bacteriocin Gr17 is effective against Listeria monocytogenes (Listeriamonocytogenes), Staphylococcus aereus (Staphylococcus aereu), Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli), Sakazaki intestinalis Enterobacter sakazakii, Enterococcus ...
Embodiment 3
[0051] Example 3 The active ingredient of Monascus CGMCC No.12502 and its application in fermented bean curd cream
[0052] For the biological characteristics and the active ingredients produced by the Monascus CGMCC No.12502 used in the present invention, see the patent "a blood fat-lowering oatmeal red yeast tea and its preparation method" (publication number: CN106173054A). The bacterium is the "M1" strain selected by the applicant's team through mutagenesis, and the preservation number is "CGMCC No. 12502". The content of Monacolin K in the fermented liquid of M1 strain detected by HPLC reached 421.7mg / L, which was 142.1% higher than that of the starting strain M1, and the performance of producing Monacolin K after 10 subcultures only decreased by 4.3%, and the genetic stability was good, indicating that M1 The strain has the characteristics of high production of functional component Monacolin K. The breeding process and characteristics of the M1 strain are detailed in th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com