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Tissue culture and rapid propagation culture medium and tissue culture and rapid propagation method for thin-bark wood

A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low proliferation rate, low reproduction rate, and inability to maintain the traits of the female parent stably, so as to reduce pollution , Improve the effect of germination rate

Active Publication Date: 2022-08-09
HENAN VOCATIONAL COLLEGE OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Thin-bark wood has many branches, bright colors, long flowering period and overflowing fragrance. It is a wild plant with great ornamental value. It can be cultivated as ornamental, greening, bonsai, etc. ability, after the thin-bark wood forms a colony or dominant species, some grasses will also grow and develop. The traditional propagation method of thin-bark wood is to use seeds or cuttings to propagate, and the germination rate of seed propagation is low and cannot stably maintain the mother For this character, the cutting propagation has less rooting and low reproduction rate, and the existing medium is used for the rapid propagation of thin-bark wood tissue culture, and there is also the problem of low multiplication rate. Plant resources are of great significance

Method used

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  • Tissue culture and rapid propagation culture medium and tissue culture and rapid propagation method for thin-bark wood
  • Tissue culture and rapid propagation culture medium and tissue culture and rapid propagation method for thin-bark wood
  • Tissue culture and rapid propagation culture medium and tissue culture and rapid propagation method for thin-bark wood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The tissue culture and rapid propagation method of thin bark wood includes the following steps:

[0028] S1, collect the well-grown thin-bark wood with axillary bud stem section, remove the leaf, and cut the twig into a small section with 2-4 axillary buds to obtain the test material;

[0029] S2, put the test material into the beaker, add 3 drops of detergent and cover the beaker with a layer of gauze, rinse with running water for 30min under the tap, then rinse with sterile water 3 times and dry;

[0030] S3. Sterilize the dried test material in alcohol with a mass concentration of 75% for 30s, rinse it with sterile water for 3 times, and then disinfect it in a NaClO solution with a mass concentration of 5% for 10min;

[0031] S4. Rinse the test material after disinfection with sterile water for 5 times, cut off both ends of the test material and connect it to the start-up medium, inoculate 30 bottles, each bottle is inoculated with 1, and count the survival rate, con...

Embodiment 2

[0036] The tissue culture and rapid propagation method of thin bark wood includes the following steps:

[0037] S1, collect the well-grown thin-bark wood with axillary bud stem section, remove the leaf, and cut the twig into a small section with 2-4 axillary buds to obtain the test material;

[0038] S2, put the test material into the beaker, add 3 drops of detergent and cover the beaker with a layer of gauze, rinse with running water for 30min under the tap, then rinse with sterile water 3 times and dry;

[0039] S3. Sterilize the dried test material in alcohol with a mass concentration of 75% for 10s, rinse it with sterile water twice, and then disinfect it in a NaClO solution with a mass concentration of 5% for 12min;

[0040] S4. Rinse the test material after disinfection with sterile water for 5 times, cut off both ends of the test material and connect it to the start-up medium, inoculate 30 bottles, each bottle is inoculated with 1, and count the survival rate, contamina...

Embodiment 3

[0045] The tissue culture and rapid propagation method of thin bark wood includes the following steps:

[0046] S1, collect the well-grown thin-bark wood with axillary bud stem section, remove the leaf, and cut the twig into a small section with 2-4 axillary buds to obtain the test material;

[0047] S2, put the test material into the beaker, add 3 drops of detergent and cover the beaker with a layer of gauze, rinse with running water for 30min under the tap, then rinse with sterile water 3 times and dry;

[0048] S3. Sterilize the dried test material in alcohol with a mass concentration of 75% for 20s, rinse it twice with sterile water, and then disinfect it in a NaClO solution with a mass concentration of 5% for 10min;

[0049] S4. Rinse the test material after disinfection with sterile water for 5 times, cut off both ends of the test material and connect it to the start-up medium, inoculate 30 bottles, each bottle is inoculated with 1, and count the survival rate, contaminati...

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Abstract

The invention belongs to the technical field of thin-bark wood tissue culture and rapid propagation, and particularly relates to a thin-bark wood tissue culture and rapid propagation culture medium and a thin-bark wood tissue culture and rapid propagation method, the thin-bark wood tissue culture and rapid propagation culture medium comprises a starting culture medium, a proliferation culture medium and a rooting culture medium, the proliferation culture medium takes the starting culture medium as a basic culture medium, and 0.5-3.0 mg of ZT is added into each liter of the basic culture medium; a rooting culture medium takes 1 / 2 MS as a basic culture medium, and 20-30 g of cane sugar, 5-7 g of agar, 0.1-0.5 mg of IBA and 0.05-0.1 mg of NAA are added into each liter of 1 / 2 MS. By utilizing the culture medium provided by the invention, the maximum multiplication coefficient can reach 6.03, and the rooting rate can reach 100%.

Description

technical field [0001] The invention relates to the technical field of tissue culture and fast propagation of Papilla spp., in particular to a medium for fast propagation of Papilla wood tissue culture and a method for fast propagation of tissue culture. Background technique [0002] It is a wild plant with great ornamental value and can be cultivated as ornamental, greening, bonsai, etc. Ability, after thin bark wood forms constructive species or dominant species, some gramineous forages will also grow and develop. The traditional propagation method of thin bark wood is to use seed propagation or cutting propagation. With this trait, the cutting propagation is less rooted, and the reproduction rate is low, and the existing medium for fast propagation of thin bark wood tissue culture also has the problem of low proliferation rate. Plant resources are of great significance. SUMMARY OF THE INVENTION [0003] In order to solve the above technical problems, the present inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/40
Inventor 郭丽朱飞雪程征张李玲侯珍珍李重赵发军杜丽娜王存纲曹海河
Owner HENAN VOCATIONAL COLLEGE OF AGRI
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