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Process for preparing porous supporter material from multiple emulsion template

A porous scaffold and multiple emulsion technology, applied in the field of preparation of biomedical materials, can solve the problems of inaccurate control of pore size and distribution, achieve good applicability, ensure mechanical strength, and improve affinity

Inactive Publication Date: 2005-01-05
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have the disadvantage of not being able to precisely control the size and distribution of pores.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Example 1: Take 3g of polyhydroxybutyrate and put it into 90ml of chloroform, stir until fully dissolved, transfer the solution to a 100ml volumetric flask after filtration, add chloroform to the mark, and obtain 100ml of polyhydroxybutyrate chloroform solution A; Dissolve 1.7g of Sorbitan trioleate Span85 in 60ml of deionized water at 70°C to obtain a stable template emulsion B; take 20ml of solution A and 0.5ml of solution B and mix together, stir until the solution is evenly mixed and stable, then let it stand for defoaming , scratch the film on the glass plate. The obtained membrane is naturally dried at room temperature, then soaked in solvent cyclohexane to remove the template, washed repeatedly with water, and dried to constant weight in a vacuum drying oven to prepare a porous monolithic membrane. Pour 2~3ml of chloroform on the tetrafluoroethylene plate to make it fully spread, lightly place the two overlapping monolithic membranes on the tetrafluoroethylene pl...

example 2

[0019] Take 1g of polyhydroxybutyrate and put it into 90ml of chloroform, stir until fully dissolved, transfer the solution to a 100ml volumetric flask after filtration, add chloroform to the mark, and obtain 100ml of polyhydroxybutyrate chloroform solution A; take sorbitan tris Dissolve 0.7g of Span85 oleate in 60ml of deionized water at 70°C to obtain a stable template emulsion B; take 20ml of solution A and 0.5ml of B and mix together, stir until the solution is evenly mixed and stable, then let it stand for defoaming, and put it on a glass plate Scratch film on. The obtained membrane is naturally dried at room temperature, then soaked in solvent cyclohexane to remove the template, washed repeatedly with water, and dried to constant weight in a vacuum drying oven to prepare a porous monolithic membrane. Pour 2~3ml of chloroform on the tetrafluoroethylene plate to make it fully spread, lightly place the two overlapping monolithic membranes on the tetrafluoroethylene plate, p...

example 3

[0021] Take 5g of polyhydroxybutyrate and put it into 90ml of chloroform, stir until fully dissolved, transfer the solution to a 100ml volumetric flask after filtration, add chloroform to the mark, and obtain 100ml of polyhydroxybutyrate chloroform solution A; take sorbitan tris Dissolve 0.1g of Span85 oleate in 60ml of deionized water at 70°C to obtain a stable template emulsion B; take 20ml of solution A and 0.5ml of B and mix together, stir until the solution is evenly mixed and stable, then let it stand for defoaming, and put it on a glass plate Scratch film on. The obtained membrane is naturally dried at room temperature, then soaked in solvent cyclohexane to remove the template, washed repeatedly with water, and dried to constant weight in a vacuum drying oven to prepare a porous monolithic membrane. Pour 2~3ml of chloroform on the tetrafluoroethylene plate to make it fully spread, lightly place the two overlapping monolithic membranes on the tetrafluoroethylene plate, p...

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PUM

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Abstract

A process for preparing porous supporter material includes such steps as putting basic material in solvent, stirring, filtering, putting template in solvent, mixing both solutions together, stirring, defoaming, taking membrane by scraping, drying in air, immersing in solvent to remove template, washing with clean water, vacuum drying to obtain single membrane, slightly pressing the membrane on tetrafluoroethylene plate with solvent layer on its surface, combining the membranes together by contacting the wet surface of one membrane with that of another membrane, and vacuum drying.

Description

technical field [0001] The invention relates to a preparation method of a biomedical material, more specifically, to a preparation method of a cell culture material for tissue engineering. Background technique [0002] Research on biomaterials has made great achievements, but biomaterials cannot replace the functions of organisms to regenerate tissues in terms of structure, function, metabolism, biochemical behavior, and biomechanical properties. Biomaterials are often regarded as "foreign bodies" by organisms. ", this is because in the past, biological materials were often designed from the perspective of materials, rather than considering how the host integrates implanted materials and rebuilds tissues from a biological perspective, so they are rejected by the body as foreign bodies. In order to meet the requirements of tissue engineering, we consider starting from the perspective of bionics, that is, to prepare bionic extracellular matrix as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L31/04A61L33/06C08J9/28
Inventor 成国祥蔡志江
Owner TIANJIN UNIV
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