Capillary column testing optical system calibration method and special device

An optical system and capillary column technology, applied in measurement devices, scientific instruments, instruments, etc., can solve the problems of beam scattering, non-negligibility, etc., and achieve the effect of strong flexibility, simple calibration, and solving the difficulty of optical calibration.

Inactive Publication Date: 2005-01-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its main problems are: (1) the cylindrical detection window seriously scatters the light beam; (2) the transparent capillary with the protective layer removed is not only used as a pool body but also as a light window. The inner diameter of the pool is not negligible compared to
Using a lens to focus also presents the optical alignment problem of how to place the capillary detection cell exactly in focus

Method used

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  • Capillary column testing optical system calibration method and special device
  • Capillary column testing optical system calibration method and special device
  • Capillary column testing optical system calibration method and special device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A capillary on-column detection cell was made by using the calibration auxiliary device (inlaid structure type in V-shaped groove), and the polymer material tube was made of polytetrafluoroethylene, combined with capillary column liquid chromatography, and benzene compounds were used as detection samples. performance of the system, the results are shown in Figure 6 . The results show that the calibration auxiliary device can effectively meet the requirements of optical calibration, and is easy to operate and has good repeatability. The chromatographic conditions are: stationary phase, KromasilC 18 (5μm); mobile phase, methanol: water = 80:20; flow rate, 8μL / min;, analytical column, 25cm×0.32mm; connecting tube and detection cell: 0.1mm i.d; Figure 6 Middle peaks 1, 2, 3, and 4 are benzene, toluene, naphthalene, and biphenyl, respectively.

Embodiment 2

[0020] Using the calibration auxiliary device (four-way structure type), a capillary on-column detection device was fabricated. The main part of the four-way cross structure is made of metal, and the polymer material is made of PEEK tube. The test conditions are the same as above. see results Figure 7 .

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Abstract

A method and device for correcting the optical system used for capillary column detection is disclosed. Said optical system uses optical fibre to transmit signals. The capillary photopool and optical fibre are respectively sleeved by the tubes with same external diameter and perpendicularly crossed and fixed in a same plane, resulting in natural vertical collimation between optical fibre and capillary photopool and natural coaxial collimation between optical fibres.

Description

Technical field: [0001] The invention relates to detection technology on a capillary column, and in particular provides a calibration method and a special device for a detection optical system on a capillary column. Background technique: [0002] Micro-column separation technology is characterized by high column efficiency, fast analysis speed, less consumption of samples and mobile phase, and good system inertness. Under normal circumstances, the inner diameter of the microcolumn is generally tens of microns to hundreds of microns, and the elution peak volume is between nanoliters and microliters. Such a small flow rate requires a detection cell with a nanoliter cell volume to match it. Studies have shown that peak broadening caused by the detection cell is acceptable as long as the detection cell volume is less than one-tenth of the chromatographic peak volume. [0003] Chromatography theory shows that the extra-column effect caused by the volume of the detection cell is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/74
Inventor 关亚风杨丙成
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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