Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Meningococcal vaccine and its preparing method

A meningococcal and vaccine technology, applied in chemical instruments and methods, antibacterial drugs, pharmaceutical formulations, etc., can solve the problems of high equipment investment requirements, no further separation, and numerous and complicated steps, so as to improve immunogenicity, Reduction of reactogenicity, effect of purification process improvement

Inactive Publication Date: 2005-07-06
BEIJING SANROAD BIOLOGICAL PROD CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high centrifugation speed required and the need for repeated density gradient centrifugation and dissolution, the equipment investment requirements are high, very cumbersome, and the steps are numerous and complicated
The product obtained was a mixture of various group B meningococcal outer membrane proteins without further isolation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Meningococcal vaccine and its preparing method
  • Meningococcal vaccine and its preparing method
  • Meningococcal vaccine and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of Group B Meningococcal Outer Membrane Protein

[0050] Kaikai Group B meningococcal strains were inoculated on a semi-comprehensive slope, expanded and transferred to the fifth-generation liquid tank for culture, and the bacteria were collected by continuous centrifugation, and 0.3-0.6M LiCl and 0.2-0.5MCH 3 The COONa mixture was used to extract outer membrane proteins. Shake at 35-45°C for 1-3 hours. Centrifuge at 4000-8000 rpm for 1 hour, collect the supernatant, then add ethanol to 60-80% to precipitate the outer membrane protein. After reconstitution with the injection solution, the 400KD filtrate was collected by ultrafiltration, and then concentrated by a 30KD ultrafiltration membrane. Use SDS-PAGE to detect the content of the desired type 1, 2 or 1, 3 outer membrane protein. Chromatography with Sephacryl S 200-400 to collect the required type 1, 2 or 1, 3 outer membrane proteins. Detection of protein content (Lowry method), main components and t...

Embodiment 2

[0052] Group A meningococcal polysaccharide conjugate

[0053] Group A meningococcal polysaccharide diluted to 4mg / ml, add cyanogen bromide to activate the polysaccharide, act at 23±3°C for 1-1.5 hours, adjust pH and maintain pH9-11, then add adipic hydrazide 2.5-5mg / ml mgps, maintain pH8-10 for 10-30 minutes, stir at 2-8°C for 10-20 hours, dialyze to remove small molecular substances, take samples to measure the residual amount of cyanogen bromide and the derivatization rate of adipic hydrazide. Add an equal amount of group B meningococcal outer membrane protein and polysaccharide to mix, add carbodiimide: 15-25mg / ml mixture, act at 5-15°C for 1-1.5 hours, and adjust pH to 5-7. The coupling stock solution was dialyzed to remove small molecular substances. 300KD membrane bag ultrafiltration is concentrated, through Sepharose CL-4B column chromatography, collects the coupling compound of high molecular weight (see Figure 4 ), sterilizing filtration to detect phosphorus conte...

Embodiment 3

[0055] Group C meningococcal capsular polysaccharide conjugate

[0056] Group C meningococcal polysaccharide diluted to 4 mg / ml, add cyanogen bromide (CNBr) to activate the polysaccharide, act at 20-30°C for 1-1.5 hours, adjust pH and maintain pH 9-11, then add adipic hydrazide (ADH) 2.5-5 mg / mg polysaccharide, maintain pH 8-10, react for 30 minutes, stir at 2-8°C for 10-20 hours, dialyze to remove small molecular substances. Sampling was carried out to measure residual CNBr content and ADH derivatization rate. Add an equal amount of group B meningococcal outer membrane protein and polysaccharide to mix, add carbodiimide (EDAC): 15-25mg / ml mixture, react at 5-15°C for 1-1.5 hours, adjust pH to 5-7. The coupling stock solution was dialyzed to remove small molecular substances, concentrated by ultrafiltration in a 300KD membrane bag, and subjected to Sepharose CL-4B column chromatography to collect high molecular weight coupling compounds (see Figure 5 ), sterilizing filtration...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a meningococcal vaccine and a preparation method thereof. The purified group A meningococcal polysaccharide and C group meningococcal polysaccharide are respectively chemically coupled with group B meningococcal outer membrane protein to form a monovalent conjugate, and then the monovalent conjugate is mixed to prepare. The vaccine can be used to prevent epidemic cerebrospinal meningitis and septicemia diseases caused by group A and C meningococci in infants and young children.

Description

technical field [0001] The present invention relates to a meningococcal vaccine. In particular, the invention relates to a meningococcal conjugate vaccine. More specifically, the present invention relates to a conjugated vaccine comprising capsular polysaccharides of group A and C meningococci coupled with outer membrane protein of group B meningococcus. The present invention also relates to the preparation method of this vaccine. Background technique [0002] Illness due to Neisseria meningitidis is an important contributor to severe illness and high mortality worldwide. Before the invention of sulfonamides and antibiotics, the fatality rate of meningococcal disease was about 70%. Today, despite good treatment, the case fatality rate of the disease can be as high as 10% even in developed countries, and the case fatality rate of sepsis caused by it can exceed 50%. Neisseria meningitidis causes 500,000 cases and 50,000 deaths worldwide each year. [0003] Neisseria menin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61P31/04C07K14/22
Inventor 范玉柱
Owner BEIJING SANROAD BIOLOGICAL PROD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com