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Production method of corn superoxide dismutase multienzyme

A dismutase multi-enzyme and superoxide technology, which is applied in the production field of corn superoxide dismutase multi-enzyme, can solve the problems of increasing product manufacturing cost, not suitable for large-scale production, and large loss of enzyme activity, so as to reduce drying cost, simplify the production process, and improve the effect of enzyme activity

Inactive Publication Date: 2005-08-17
王建英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem that exists when utilizing this method to produce superoxide dismutase multienzyme is: 1, because this method is with the mixture of salts such as phosphate, sodium salt, potassium salt and fresh water as extracting solution, thereby on the one hand a large amount of It consumes increasingly scarce fresh water resources. On the other hand, when removing the salt contained in the semi-finished product, at least two salt precipitation processes are required, so the process is relatively complicated. Finally, when the water-salt mixture is discharged, it should be purified first. , and then discharge, it will inevitably increase the manufacturing cost of the product, if it is not purified and discharged directly, it will pollute the environment; 2, in the process of drying the product, the method adopts freeze drying or spray drying technology, the former cost Higher, the latter has a greater loss of enzyme activity, neither of which is suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A. Prepare materials

[0030] Prepare 300kg of raw corn;

[0031] B. Remove impurities and soak

[0032] Remove the impurities in the raw corn, then soak the corn in circulating tap water for 8 hours, and then take it out for later use;

[0033] C, activation, germination

[0034] Soak the corn soaked in process B in a red enzyme solution with a concentration of 0.01% and a temperature of 30°C for 2 hours, then take out the corn and put it in a germination room with an indoor temperature of 30°C. Store it naturally for 15 hours to germinate it, then take it out for later use;

[0035] D. pulping

[0036] Adding ethanol with a concentration of 20% to the germinated corn, and making the weight ratio of the germinated corn and ethanol 1:4, and then mixing and pulverizing the germinated corn and ethanol into corn steep liquor by using a pulverizer;

[0037] E. Filter sterilization

[0038] After the corn syrup is stirred evenly, filter with a primary filter, remove th...

Embodiment 2

[0044] A. Prepare materials

[0045] Prepare 300kg of raw corn;

[0046] B. Remove impurities and soak

[0047] Remove the impurities in the raw corn, then soak the corn in circulating tap water for 3 hours, and then take it out for use;

[0048] C, activation, germination

[0049] Soak the corn soaked in process B in a red enzyme solution with a concentration of 0.03% and a temperature of 26°C for 2 hours, then take out the corn and put it in a germination room with an indoor temperature of 26°C. Natural storage for 24 hours to germinate, then take out for later use;

[0050] D. pulping

[0051] Adding ethanol with a concentration of 55% to the germinated corn, and making the weight ratio of the germinated corn and ethanol 1:2, then using a pulverizer to mix and grind the germinated corn and ethanol into corn syrup, and then taking it out for use;

[0052] E. Filter sterilization

[0053] After the corn syrup is stirred evenly, filter with a primary filter, remove the f...

Embodiment 3

[0059] A. Prepare materials

[0060] Prepare 300kg of raw corn;

[0061] B. Removal of impurities

[0062] Remove the impurities in the raw corn, then soak the corn in circulating tap water for 5 hours, and then take it out for later use;

[0063] C, activation, germination

[0064] Soak the corn soaked in process B in a red enzyme solution with a concentration of 0.02% and a temperature of 23°C for 6 hours, then take out the corn and put it in a germination room with an indoor temperature of 25°C. Store it naturally for 20 hours to germinate it, then take it out for later use;

[0065] D. pulping

[0066]Adding ethanol with a concentration of 60% to the germinated corn, and making the weight ratio of the germinated corn and ethanol 1:3, and then using a pulverizer to mix and grind the germinated corn and ethanol into corn syrup, and then taking it out for use;

[0067] E. Filter sterilization

[0068] After the corn syrup is stirred evenly, filter with a primary filter,...

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PUM

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Abstract

A process for preparing corn superoxide dismutase (SOD) multienzyme includes such steps as preparing raw materials, removing impurities, immersing, activating, germinating, pulping, filtering to remove bacteria, recovering alcohol, and drying. Its advantages are low cost, high enzyme activity and less consumption of water.

Description

technical field [0001] The invention relates to a production method of superoxide dismutase multi-enzyme, specifically a production method of corn superoxide dismutase multi-enzyme. Background technique [0002] The superoxide dismutase multienzyme refers to the mixture of superoxide dismutase, glutathione peroxidase and catalase. The main effect of this mixture on the human body is to effectively remove superoxide anion-free radicals in the body, reduce cell damage or death in the body, thereby reducing the occurrence of diseases and delaying aging. Existing methods for producing superoxide dismutase multienzymes mainly contain two types, one is to extract from animal viscera, and the other is to extract from plants such as corn, and the method for extracting superoxide dismutase from corn is mostly The enzymatic method is mainly "salting out method". This method is recorded in many books and periodicals, for example, in the biochemistry course co-edited by Shen Tong and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02
Inventor 王建英
Owner 王建英