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Jojoba tissue cultivation quick breeding method

A rapid and basic medium technology, applied in the field of artificial propagation and cultivation of plants, can solve the problems of serious glass seedlings, long rooting time, low multi-bud rate, etc., to save land for seedlings, reduce production costs, and stabilize genetic traits Effect

Inactive Publication Date: 2005-12-28
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Guangxi, Guangdong, Yunnan and other provinces and districts have a small number of introduced species, and there are few reports about the shoot tip propagation of jojoba adult plants and seedlings, and there are many problems, mainly in: low multi-bud rate, serious glass seedlings, easy Browning, poor rooting, and long rooting times
Thereby seriously restricting the large-scale popularization of jojoba in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Take the stem tip of the jojoba plant material, wash it with running water for 8-12 hours, absorb excess water with filter paper, then disinfect it with 0.1% mercury dichloride for 3-5 minutes, and rinse it with sterile water until clear.

[0016] 2. Trim the material washed to clear to more than 0.3 cm, and inoculate it into the starting medium of the present invention. The composition ratio of the starting medium is: 200 mg / liter of hydrolyzed milk protein is added to the 4736.43 mg / liter MS basic medium , 6-furfuryl purine 2 mg / L, 6-benzyl purine 0.5 mg / L, edible sugar 25 g / L, and the balance of water. Place the inoculation chamber for light cultivation.

[0017] 3. Cut the test-tube plantlets cultivated more than 3 centimeters into stems of more than 0.5 centimeters respectively, and inoculate them into the proliferation medium of the present invention. The composition ratio of the proliferation medium includes adding inositol in 4736.43 mg / liter MS basic medium ...

Embodiment 2

[0021] 1. Take the stem section of the jojoba plant material, rinse it with running water for 12-24 hours, absorb excess water with filter paper, then routinely disinfect it with 0.1% mercury dichloride, and rinse it with sterile water until clear.

[0022] 2. Trim the material washed to clear to more than 0.3 centimeters. Inoculate into the starting medium of the present invention, the composition ratio of starting medium is: add hydrolyzed milk protein 200 mg / liter in 4736.43 mg / liter MS basic medium , 1 mg / L of 6-furfuryl purine, 0.5 mg / L of 6-benzyl purine, 25 g / L of edible sugar, and the remainder of water were placed in the inoculation room for light cultivation.

[0023] 3. Cut the test-tube plantlets cultivated more than 3 centimeters into stem sections more than 0.5 centimeters respectively. Inoculate in the proliferation medium of the present invention, the composition ratio of the proliferation medium is: add muscle in 4736.43 mg / liter MS basic medium Alcohol 500 mg...

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PUM

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Abstract

The invention provides a jojoba tissue culture rapid propagation method for industrialized production to meet market demand. The tissue culture steps of jojoba include A. Disinfection of shoot tip; B. Initiation culture; C. Proliferation culture; D. Rooting culture. Propagation of jojoba by means of plant tissue culture can not only save a lot of foreign exchange, but also not be affected by external conditions. It can be carried out in all seasons, saves the area occupied by seedlings, reduces production costs, and preserves all the excellent traits of the mother, and the genetic traits are stable. . This method can form a large number of excellent test-tube seedlings in a short period of time, and carry out large-scale and factory production.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of jojoba, and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Jojoba (Simmondisa Chinesis), English name Jojoba, Jojoba family, Jojoba genus. Native to Mexico and California, dioecious. The content of jojoba oil in the seeds is more than 50%. The main components are unsaturated higher alcohols and fatty acids, which have good stability and are easy to integrate with the skin. It has the characteristics of anti-oxidation and high temperature resistance. It is a high-grade lubricating oil in the high-tech field and a raw material for the manufacture of medicine and cosmetics. It is called "renewable resources in the 21st century" and "plant gold". Jojoba mainly relies on imported seeds for reproduction in my country, and its fecundity is very low. At present, Guangxi, Guangdong, Yunnan and other provinces and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王玉珍罗景兰徐进
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI