Process for preparing chromium-enriched beer yeast
A brewer's yeast and preparation technology, which is applied in the field of chromium-rich brewer's yeast fermentation technology, can solve the problems of long process flow, large reagent consumption, high concentration, etc., and achieve the goal of improving metabolism-dependent adsorption, increasing GTF content, and simplifying the operation process Effect
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Embodiment 1
[0023] Embodiment 1. Pick a Brewer yeast (saccharomyces cervisiae) single colony and carry out secondary strain cultivation in wort medium, seed culture condition: prepare 50% wort by wort: ultrapure water=1: 1, pH The value is 4.5-5.5, the temperature is 27°C-29°C, the shaking culture time of 200rpm is 20-24 hours for the first and second level, and the first level is cultivated to OD 600 ≥2.0, secondary culture to OD 600 ≥4.0, the inoculum size of secondary culture is 10%. Fermentation conditions in the tank: the initial volume of the fermented liquid is 18 liters, 100% wort medium, the secondary seeds are transferred to the medium according to the amount of 10% of the planting amount, and the pH value is adjusted to 6.0 with a concentration of 28% ammonia water; Gradient feeding of Cr after 10 hours 3+ ,, that is, in the growth stage, after inoculation, adjust the rotational speed and ventilation, so that the dissolved oxygen DO≥20%, adjust the pH value to 5.6-6.0, and th...
Embodiment 2
[0026] Example 2. The difference between this example and Example 1 is that 0.1N HCl is used to adjust the pH value to 5.5-5.6 during the chromium enrichment culture stage of fermentation. After the yeast grows and cultures for 9 hours, it is cultured for 8 hours, and then the wort is added. Continue to culture for 1 hour to OD 600 After ≥9.0, the gradient addition method is used to add chromium salt solution until Cr 3+ The concentration is 0.05, 0.10, 0.15, 0.20g / l fermentation broth, stop adding Cr 3+ ,, all the other conditions are identical with embodiment 1. The enrichment effect of chromium in brewer's yeast is listed in Table 2.
[0027] Cr 3+ Concentration g / L
[0028] The data in Table 2 show that in Cr 3+ When the concentration is 0.10-0.15 g / L, the total chromium in the dry bacterial powder cell reaches 539.4-673.4 μg / g dry yeast, and the chromium-enriched yeast powder washed with ultrapure water and dried is extracted with 0.1mol / l ammonia water, fr...
Embodiment 3
[0029] Embodiment 3. The difference between this embodiment and Example 2 is that after the yeast was grown and cultivated for 11 hours, it was cultivated for 8 hours, and then the wort was added to continue to cultivate for 3 hours to OD 600 ≥9.0, then use the gradient addition method to add chromium salt solution until Cr 3+ Concentration is 0.15g / l fermented liquid, total fermentation time is 30, 35, 40, 45, 50, 55 hours, all the other conditions are identical with embodiment 2. The enrichment effect of chromium in brewer's yeast is listed in Table 3.
[0030] time
[0031] The data in Table 3 shows that when the fermentation time is 40 to 50 hours, the total chromium in the dry bacterial powder cells reaches 670.1 to 689.2 micrograms per gram of dry yeast. l Extracted with ammonia water and freeze-dried to obtain biologically active chromium GTF, the content of which reaches 298.6-318.2 micrograms per gram of dry yeast, and the yield of wet bacteria reaches 43....
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