Process for preparing serine-rich protein employing cysteine synthase (cysk) gene

A technology of cysteine ​​and serine, which is applied in the field of protein preparation, can solve the problems of no results, difficulty in improving the yield of heterogeneous protein preparation, and a large amount of time
CN1269965CInactive Publication Date: 2006-08-16KOREA ADVANCED INST OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
KOREA ADVANCED INST OF SCI & TECH
Publication Date
2006-08-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a process for preparing a foreign protein comprising culturing a bacterium containing the cysteine synthase (cysK) gene and a gene encoding the foreign protein. The present invention comprises the steps of culturing a bacterium transformed with an expression vector containing a gene encoding a serine-rich foreign protein and an expression vector containing the cysK gene, or a bacterium transformed with an expression vector containing the cysK gene and a gene encoding a serine-rich foreign protein and isolating the foreign protein therefrom. The present invention is expected to be widely used to increase the production yield of a serine-rich foreign protein.
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Description

technical field

[0001] The invention relates to a preparation method of serine-rich protein, which comprises culturing bacteria containing cysteine ​​synthetase (cysK) gene and heterologous protein gene. More specifically, the present invention relates to a method for preparing a serine-rich protein, which comprises culturing a bacterium containing both a serine-rich heterologous protein gene and a cysteine ​​synthase (cysK) gene, and isolating a bacterium rich in Serine-containing heterologous proteins. Background technique

[0002] E.coli is a commonly used strain for the synthesis and preparation of heterologous proteins. This strain is used to produce proteins such as interferon, interleukin 2, colony-stimulating factor, growth hormone, insulin-like growth factor and human serum albumin through recombinant technology. . In order to efficiently prepare heterologous proteins in E.coli, plasmid vectors expressing heterologous proteins, suitable culture conditions, conditi...

Claims

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