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Method for rapidly breeding citrange

A Citrus aurantium, rapid technology, applied in the field of grafting plants as explants and continuous subculture proliferation culture, can solve the problems of poor bud differentiation quality, callus, and trait variation, and achieve the effect of good quality.

Inactive Publication Date: 2007-01-10
邓子牛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In terms of rapid propagation of citrus in vitro culture, most of them use seedling hypocotyls and cotyledon cultures at present, and only a few of them are collected from grafted tree stem tips or stem segments as explants. This is mainly because the differentiation quality of buds is poor, and the differentiation coefficient Low, easy to produce callus during the culture process, especially in the process of bud proliferation and culture, callus tends to form seedlings, often produces trait variation, and the problem of unstable seed quality occurs, which cannot reach the industrialized breeding of citrus seedlings. skills requirement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] (1) Induced germination of explant buds Take a single-node stem section with buds as an explant to induce the germination of buds. After the branches are defoliated and thorned, they are cut into 5 cm long stem sections, rinsed with tap water for 60 minutes, and placed on an ultra-clean workbench. Disinfect the surface with 70% alcohol for 1 min, then disinfect with 0.1% mercuric acid solution for 12 min, pour off the mercuric solution, and rinse with sterile water for 3 to 4 times; Cut into 1.5cm-long single-segmented stems with buds with a scalpel, then put the buds on the medium with the eyes facing up, and cultivate them; the medium formula is MS+BA 1mg·L -1 , the culture temperature is 24±2°C, the light is continuously illuminated for 12 hours every day, and the light intensity is 1200lx. After 7 days of inoculation, all the buds germinate, and the buds are neat, strong and fast growing, and can reach a height of 1.5-2cm in 15 days.

[0011] (2) Proliferation and ...

Embodiment 2

[0014] (1) Induction of germination of explant buds The induction medium formula is MS+BA 0.5mg L -1 , other conditions are the same as in Example 1, all the buds germinate after 10 days of inoculation, and can reach a height of 1.5 to 2 cm in 20 days; (2) The proliferation and subculture of buds The subculture medium is MS+BA 0.5mg L -1 +NAA 0.02mg·L -1 , bud month multiplication coefficient 2.86; (3) Rooting culture and transplanting Select the vigorous shoots from the tender shoots of multiplication culture, and inoculate them into the rooting medium (1 / 2MS+NAA 0.5mg L -1 +0.2% activated carbon) for rooting culture; the rooting rate can reach 95%; after cultivating for 25 days, take out the test-tube seedlings, wash the culture medium, and transfer to the culture pot that white sand and fermented cottonseed hulls (1:1) are housed, use Covered with plastic film and hardened in the greenhouse, the survival rate is 92%.

Embodiment 3

[0016] The induced germination of explant buds and the subculture of bud proliferation are the same as in Example 1, and the rooting medium is changed to 1 / 2MS+NAA 0.2mg L -1 +0.2% activated carbon, the rooting rate is 63%. After cultivating for 30 days, take out the test-tube seedlings, wash the culture medium, transfer to the culture pot that white sand and fermented cottonseed hulls (1:1) are housed, cover with plastic film, The seedlings are hardened in the greenhouse, and the survival rate is 85%.

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Abstract

The present invention discloses a quick propagation method of citrange. Said method includes the following steps: (1) induced germination and induced culture of graft plant with nodal stem part explant bud, its induced culture medium formula is MS+BA 0.5-1.5mg.L(-1); (2) propagation subculture of bud, its culture medium is MS+BA 0.2-1mg.L(01)+NAA 0.01-0.1mg.L(-1); (3) rooting culture and transplantation, using 1 / 2 MS+NAA 0.2-0.5mg.L(-1)+0.2% active carbon to induce propagation bud to root, its culture temp, is 24 plus or minus 2dec.C, continuous light treatment for 10-14hr. per day, light intensity is 1000-1500Lx, culture for 20-35 days, taking out test tube seedling and making transplantation. The transplantation matrix formula is white sand: turf or fermented cotton seed peel+1:0.8-1.2.

Description

technical field [0001] The invention relates to a method for rapidly propagating citrange, in particular to a method for using grafted plants as explants and continuous subculture multiplication and culture. Background technique [0002] Citrus [C.sinensis(L.)Osb×Poncirus trifoliate(L.)Raf.] is a hybrid of sweet orange and Citrus trifoliate. It is a rootstock widely used in citrus producing countries in the world. The shell is resistant to foot rot and recession diseases, and has the characteristics of drought tolerance, cold resistance, barren resistance, wide adaptability to soil, and strong affinity with most commercial varieties. [0003] In terms of rapid propagation of citrus in vitro culture, most of them use seedling hypocotyls and cotyledon culture at present, and only a few of them are collected from grafted tree stem tips or stem segments as explants. This is mainly because the differentiation quality of buds is poor, and the differentiat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邓子牛胡春华
Owner 邓子牛
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