Method for extracting rice protein by alkaline process

A rice protein, alkaline extraction technology, applied in the direction of plant peptides, peptide sources, etc., can solve the process parameters such as NaOH concentration extraction time, solid-liquid ratio is not reasonable, there is no industrial production method of alkaline extraction of rice protein, alkali It is difficult to apply the method to industrial production and other problems to achieve good extraction effect, low cost and low production cost.

Inactive Publication Date: 2007-02-07
GAEA GEM RICE
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the alkali extraction method is used to extract rice protein, which is still in the theoretical research stage at home and abroad, and there is no report on the industrial production method of alkali extraction of rice protein.
Tracing it to its cause is mainly because protein content is low (8%-12%) in the rice, needs to consume a large amount of alkali and water when extracting, and the protein concentration in the extract is very low, and its cost is higher, simultaneously, because existing bibliographical information Some process parameters of the medium-alkaline extraction method, such as NaOH concentration, extraction time, solid-liquid ratio, etc., are not reasonable, which makes it difficult to apply the alkaline extraction method to industrial production

Method used

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  • Method for extracting rice protein by alkaline process
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Examples

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Effect test

Embodiment 1

[0037] Get 500 grams of early indica rice, add 2.58 liters of 0.09mol / L NaOH solution, soak for 2 hours and refine to 100 mesh or more and stir for 2 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 5.5 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at a high speed of 4000 rpm for 15 minutes, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. P...

Embodiment 2

[0039] Get 500 grams of early indica rice, add 3 liters of 0.09mol / L NaOH solution, soak for 2.5 hours and grind to more than 100 mesh and stir for 2.5 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 5.0 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at a high speed of 4000 rpm for 15 minutes, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. ...

Embodiment 3

[0041] Get 500 grams of early indica rice, add 3.5 liters of 0.09mol / L NaOH solution, soak for 3 hours and grind to more than 100 mesh and stir for 3 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 4.5 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at a high speed of 4000 rpm for 15 minutes, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. P...

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Abstract

The invention discloses a method to rice protein by alkali, including the technical step in sequence: rice washing, lye soaking, slurry milling, stir-extracting, separating, acid precipitation, separating and drying; NaOH solution soaking: concentration 0.05-0.15mol / L, solid-liquid ratio 1 to 6-8 and soaking time for 2-3 hours; the stir-extracting is to stir by keeping mixed liquor pH 11, the acid precipitation is isoelectric precipitation, i.e. it adjusts pH value by HCl to 5.5 to precipitate protein; the drying temperature is limited below 50 deg.C. It can continuously extract rice protein on a large scale. Its extraction rate is high and its fat content is low.

Description

technical field [0001] The invention relates to a method for extracting rice protein from rice, in particular to a method for extracting rice protein by alkaline method. Background technique [0002] The most widely used vegetable protein is soybean protein (protein content in soybean: 42%). However, it has recently been found that rice protein has hypoallergenic properties that other proteins do not have, and is more suitable for human consumption, especially for infants. In addition, rice protein has excellent nutritional value, its amino acid ratio is relatively reasonable, close to complete protein, and its biological value (BV) and protein value (PV) are also higher than other vegetable proteins, making it the top grade among vegetable proteins. Therefore, there is a great demand for rice protein both at home and abroad, and our country regards the extraction of rice protein as a national "Tenth Five-Year Plan" research plan. [0003] The extraction of soybean protein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/14A23J1/12
Inventor 孙庆杰田正文殷七荣陈小延
Owner GAEA GEM RICE
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