Effective site extract of lotus plumule and its usage
A technology of effective parts and extraction methods, applied in the field of extraction of effective parts of lotus seed heart, can solve problems that have not yet been seen, and achieve the effects of regulating insulin sensitivity, reducing the number of islet cells, and increasing the number of islet cells
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Embodiment 1
[0035] Example 1. A method for extracting effective parts of lotus seed heart, the steps are as follows,
[0036] (1) taking the lotus seed heart and extracting it with 60% ethanol under reflux, merging the ethanol extract, and concentrating under reduced pressure;
[0037] (2) After concentrating the extract, add hydrochloric acid to adjust the pH to 4, and centrifuge to obtain the supernatant;
[0038] (3) adding ammonia water to the supernatant to adjust the pH to 9, then adding 10% sodium chloride, cooling at a low temperature below 10 degrees Celsius, and centrifuging to obtain a precipitate;
[0039] (4) The precipitate is dried under reduced pressure and crushed to obtain the extract of the effective part of the lotus seed heart.
Embodiment 2
[0040] Example 2. A method for extracting effective parts of lotus seed heart, the steps are as follows,
[0041] (1) take the lotus seed heart medicinal material and extract it by percolating with 70% ethanol, combine the ethanol extract, and concentrate under reduced pressure;
[0042] (2) After concentrating the extract, add sulfuric acid to adjust the pH to 4.5, and centrifuge to obtain the supernatant;
[0043] (3) Add milk of lime to the supernatant to adjust the pH to 9.5, then add 15% ammonium chloride, cool at a low temperature below 10 degrees Celsius, and centrifuge to obtain a precipitate;
[0044] (4) The precipitate is dried under reduced pressure and crushed to obtain the extract of the effective part of the lotus seed heart.
Embodiment 3
[0045] Example 3. A method for extracting effective parts of lotus seed heart, the steps are as follows,
[0046] (1) taking the lotus seed heart medical material and using concentration to be 80% ethanol impregnation extraction, merging the ethanol extract, concentrating under reduced pressure;
[0047] (2) After concentrating the extract, add citric acid to adjust the pH to 5, and centrifuge to obtain the supernatant;
[0048] (3) Add milk of lime to the supernatant to adjust the pH to 10, then add 20% ammonium chloride, cool at a low temperature below 10 degrees Celsius, and centrifuge to obtain a precipitate;
[0049] (4) The precipitate is dried under reduced pressure and crushed to obtain the extract of the effective part of the lotus seed heart.
[0050] It can be used to prepare medicines for preventing and treating syndrome X with insulin resistance IR as the basic pathology.
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