Microwave assisted non-aqueous phase enzyme catalytic reacting device

Abstract

This invention relates to a microwave assistance non aqueous enzyme catalytic reaction device, which belongs to chemical reaction equipment field. The device uses the magtron of common microwave oven as the adjustable microwave emitting source, installing the enzyme reaction vessel in the microwave barrier cavity, stirring by magnetic force and air, combining with the microwave barrier temperature sensor self-feedback controlling system, which is a composite set applying maser radiation and non aqueous phase enzyme catalysis in the catalytic organic reaction. The device can make the reaction processing in the range which confines 1 Deg. C off the setting temperature, and the reacting material can mix evenly and quickly; in addition, it can sample on-line in the uninterrupted maser radiation, so it can reflect the character of non aqueous phase enzyme catalysis in the microwave field.

Description

technical field

[0001] A microwave-assisted non-aqueous enzyme catalytic reaction device, the invention belongs to the technical field of chemical reaction devices. Background technique

[0002] Microwave-assisted non-aqueous enzyme catalysis is a new synthesis technology that uses microwave radiation and non-aqueous enzyme catalysis to catalyze organic reactions. The thermal and non-thermal effects of microwaves enable this technology to achieve what a single catalytic method cannot achieve. catalytic effect.

[0003] The existing domestic microwave reaction devices do not have temperature control units and stirrers suitable for enzymatic reactions. Their temperature control principle is to control the reaction temperature by adjusting the microwave power or intermittent microwave heating, so they cannot be used to study and make full use of microwaves. In addition, the mechanical stability of the enzyme catalyst, the uniform dispersion of the reaction system and the safet...

Examples

Embodiment 1

[0015] Example 1: Microwave-assisted non-aqueous enzyme catalyzed reaction device

[0016] A magnetron 2 is placed in the microwave shielding cavity 1 as an adjustable microwave emission source, and a glass reaction vessel 3 is built in the microwave shielding cavity, the reaction vessel has a jacket 4, and the reaction vessel is placed on a magnetic stirrer 5 , the magnetic stirrer is placed at the bottom of the microwave shielding cavity, and two circular holes 6 and 7 are opened on the back or side of the microwave shielding cavity as the inlet and outlet interfaces of the cooling medium. The cooling medium to be connected has a round hole on the upper part of the microwave shielding cavity, that is, the upper microwave shielding interface 8, facing the reaction vessel mouth, and a microwave shielding temperature sensor 9, an on-line sampling tube 10, a device or No gas inlet tube 11 is installed, and all openings on the microwave shielding cavity are equipped with metal sl...

Embodiment 2

[0018] Embodiment 2: microwave-assisted enzymatic synthesis of amyl octanoate

[0019] Put 3.6g of n-octanoic acid, 2.2g of n-pentanol and 0.06g of distilled water in the glass jacketed reaction vessel of the microwave reaction device, turn on the magnetic stirring to mix the reaction system evenly, set the microwave power to 200w, and set the reaction temperature to 50 ℃. Turn on the microwave reaction device, add 200 mg of immobilized lipase LRI after the reaction temperature rises to 50° C., and start timing. At different stages of the reaction, samples are taken from the sampling port of the device to measure the initial velocity of the reaction. The initial velocity of the microwave-assisted non-aqueous enzyme-catalyzed reaction was 4.2mmol / min·g; while other reaction conditions were the same, the initial velocity of the reaction in the conventional heating mode was only 1.8mmol / min·g. Microwave radiation significantly increased the initial velocity of the reaction.

Embodiment 3

[0020] Embodiment 3: microwave-assisted enzymatic synthesis of butyl octanoate

[0021] Add 1.44g n-octanoic acid, 0.74g n-butanol, 0.02g distilled water and 15mL tetrahydrofuran solvent into the glass jacketed reaction vessel of the microwave reaction device, turn on the magnetic stirring to mix the reaction system evenly, set the microwave power to 400w, and set the reaction The temperature was 55°C. Turn on the microwave reaction device, add 100 mg of immobilized lipase LRI after the reaction temperature rises to 55° C., and start timing. At different stages of the reaction, samples are taken from the sampling port of the device to measure the initial velocity of the reaction. The initial velocity of the microwave-assisted non-aqueous enzyme-catalyzed reaction was 0.76mmol / min·g; while other reaction conditions were the same, the initial velocity of the reaction in the conventional heating mode was only 0.25mmol / min·g. Microwave radiation significantly increased the initi...

technical field

[0001] A microwave-assisted non-aqueous enzyme catalytic reaction device, the invention belongs to the technical field of chemical reaction devices. Background technique

[0002] Microwave-assisted non-aqueous enzyme catalysis is a new synthesis technology that uses microwave radiation and non-aqueous enzyme catalysis to catalyze organic reactions. The thermal and non-thermal effects of microwaves enable this technology to achieve what a single catalytic method cannot achieve. catalytic effect.

[0003] The existing domestic microwave reaction devices do not have temperature control units and stirrers suitable for enzymatic reactions. Their temperature control principle is to control the reaction temperature by adjusting the microwave power or intermittent microwave heating, so they cannot be used to study and make full use of microwaves. In addition, the mechanical stability of the enzyme catalyst, the uniform dispersion of the reaction system and the safet...

Examples

Embodiment 1

[0015] Example 1: Microwave-assisted non-aqueous enzyme catalyzed reaction device

[0016] A magnetron 2 is placed in the microwave shielding cavity 1 as an adjustable microwave emission source, and a glass reaction vessel 3 is built in the microwave shielding cavity, the reaction vessel has a jacket 4, and the reaction vessel is placed on a magnetic stirrer 5 , the magnetic stirrer is placed at the bottom of the microwave shielding cavity, and two circular holes 6 and 7 are opened on the back or side of the microwave shielding cavity as the inlet and outlet interfaces of the cooling medium. The cooling medium to be connected has a round hole on the upper part of the microwave shielding cavity, that is, the upper microwave shielding interface 8, facing the reaction vessel mouth, and a microwave shielding temperature sensor 9, an on-line sampling tube 10, a device or No gas inlet tube 11 is installed, and all openings on the microwave shielding cavity are equipped with metal sl...

Embodiment 2

[0018] Embodiment 2: microwave-assisted enzymatic synthesis of amyl octanoate

[0019] Put 3.6g of n-octanoic acid, 2.2g of n-pentanol and 0.06g of distilled water in the glass jacketed reaction vessel of the microwave reaction device, turn on the magnetic stirring to mix the reaction system evenly, set the microwave power to 200w, and set the reaction temperature to 50 ℃. Turn on the microwave reaction device, add 200 mg of immobilized lipase LRI after the reaction temperature rises to 50° C., and start timing. At different stages of the reaction, samples are taken from the sampling port of the device to measure the initial velocity of the reaction. The initial velocity of the microwave-assisted non-aqueous enzyme-catalyzed reaction was 4.2mmol / min·g; while other reaction conditions were the same, the initial velocity of the reaction in the conventional heating mode was only 1.8mmol / min·g. Microwave radiation significantly increased the initial velocity of the reaction.

Embodiment 3

[0020] Embodiment 3: microwave-assisted enzymatic synthesis of butyl octanoate

[0021] Add 1.44g n-octanoic acid, 0.74g n-butanol, 0.02g distilled water and 15mL tetrahydrofuran solvent into the glass jacketed reaction vessel of the microwave reaction device, turn on the magnetic stirring to mix the reaction system evenly, set the microwave power to 400w, and set the reaction The temperature was 55°C. Turn on the microwave reaction device, add 100 mg of immobilized lipase LRI after the reaction temperature rises to 55° C., and start timing. At different stages of the reaction, samples are taken from the sampling port of the device to measure the initial velocity of the reaction. The initial velocity of the microwave-assisted non-aqueous enzyme-catalyzed reaction was 0.76mmol / min·g; while other reaction conditions were the same, the initial velocity of the reaction in the conventional heating mode was only 0.25mmol / min·g. Microwave radiation significantly increased the initi...

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