Method for the purification, recovery, and sporulation of cysts and oocysts

Abstract

A vaccine for in ovo vaccination against avian coccidiosis produced by a method including obtaining the coccidial oocysts from a fecal suspension, homogenizing the fecal suspension, separating the oocysts from the fecal debris by either salt flotation using sodium sulfate or gas flotation using air, sporulating the oocysts using hydrogen peroxide and air sparging, bleaching the sporulated oocysts, washing the bleached oocysts, concentrating the sterile washed oocysts and combining the concentrates of various species of coccidial oocysts, and producing a vaccine. The method in whole or in part can be applied to other kinds of encysted protozoa to produce vaccines for various types of animals.

Description

Background of the invention [0001] The present invention generally relates to a method for purifying, recovering and sporulating cystic protozoa, known pathogens that attack the gastrointestinal tract of a host, for use in vaccine production. When the host's immune system is weak or suppressed, such as in very young, very old, and immunocompromised hosts, infection can be fatal, and this loss can also translate into economic loss. Protozoa exist in the form of relatively stable cysts in the natural environment, and when the protozoa is a sporozoite, it is also called oocyst. When ingested into the host, the encapsulated protozoa react to the conditions of the gastrointestinal tract and infect the host. [0002] In order to prevent or alleviate such diseases, non-in ovo vaccines have been developed. Such vaccines generally expose the host to low levels of the protozoa to immunize the host without causing disease. Such vaccines include: a vaccine against avian coccidiosis cau...

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