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Environment stress effect promoter

A promoter and environmental technology, applied in the direction of application, angiosperm/flowering plants, biochemical equipment and methods, can solve problems such as stress tolerance that has not been fully elucidated

Inactive Publication Date: 2002-10-09
RIKEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it does not fully elucidate how overexpression of the DREB1A cDNA molecule in transgenic plants increases stress tolerance to severe cold, drought and salt

Method used

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  • Environment stress effect promoter
  • Environment stress effect promoter
  • Environment stress effect promoter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1. Isolation of promoters 1. Materials and methods (1) Arabidopsis cDNA clone

[0110] To prepare a microarray, a total of about 1,300 cDNA molecules were used, which were genes isolated from Arabidopsis full-length cDNA library, RD (response to dehydration) gene, ERD (early response to dehydration) gene, kin1 gene, kin2 gene, cor15a gene, and α-tubulin gene as internal standards, and mouse acetylcholine nicotinic receptor ε subunit (nAChRE) gene and mouse glucocorticoid receptor as negative controls homologous genes. Positive control: Drought-induced genes (responsive gene to dehydration: rd, and early responsive gene to dehydration: erd) Internal standard: α-tubulin gene Negative control: For analysis of non-specific hybridization, acetylcholine nicotine receptor The human ε subunit (nAChRE) gene and the homologous gene of the mouse glucocorticoid receptor have essentially no homology to any sequence given in the Arabidopsis database.

[0111] Furthermore, i...

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PUM

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Abstract

The present invention provides a stress response promoter. The environmental stress response promoter of the present invention comprises the DNA of the following (a), (b) or (c); (a) is composed of any nucleotide sequence selected from sequence numbers: 1 to 18; (b) consists of Containing a nucleotide sequence with deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 18 and having the function of serving as an environmental stress response promoter and (c) hybridize under stringent conditions to a DNA consisting of any nucleotide sequence selected from SEQ ID NO: 1 to 18, and has a DNA that acts as an environmental stress response promoter.

Description

technical field [0001] The present invention relates to an environmental stress response promoter. Background technique [0002] With the help of gene sequencing programs, a large number of genome and cDNA sequences of many organisms have been determined, and in a plant model Arabidopsis thaliana, the complete genome sequence of two chromosomes has been determined (Lin, X. et al. , (1999) Nature 402, 761-768; Mayer, K. et al. (1999) Nature 402, 769-777). [0003] The EST (Expressed Sequence Tag) scheme also contributes greatly to the discovery of expressed genes (Hofte, H. et al., (1993) Plant Journal 4, 1051-1061; Newman, T. et al., (1994) Plant Physiology 106, 1241- 1255; Cooke, R. et al. (1996) Plant J. 9, 101-124, Asamizu, E. et al. (2000) DNA Res. 7, 175-180). For example, dbEST, the EST database of the National Center for Biotechnology Information (NCBI), includes partial cDNA sequences that describe more than half (approximately 28,000 genes) of all gene complements...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N5/10C12N15/09C12N15/11C12N15/29C12N15/82
CPCA01K2217/05C12N15/8237C12N15/8273
Inventor 筱崎一雄关原明楠城时彦
Owner RIKEN