Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for separating and extracting total flavone from goldenrain tree plant and its application

A technology of euanone and total flavonoids, which is applied to the separation and extraction of total flavonoids from eucalyptus plants and its application field, which can solve the problems of limited development

Inactive Publication Date: 2002-11-20
林文翰
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Song YN et al. reported in Chang C.J., J.Nat.Prod., 1994, 57 (12), p.1670, isolated from the weakly polar part of Taiwan eucalyptus alcohol extract to 3 kinds of in vitro effects on human lung cancer (A-549 ), human breast cancer (MCF-7) and colorectal adenocarcinoma (HT-29) and other 8 cell lines have cytotoxic and microtubule polymerase inhibitory cyclolignin compounds, which are similar to anti-cancer podophyllotoxin, However, they are all fat-soluble lignin compounds, and their development is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The method and steps of separating the total flavonoids of eucalyptus in advance are:

[0054] 1. Take 1 kg of sun-dried raw material eucalyptus branches and leaves, crush them into 20 meshes with a pulverizer, put them into a 20-liter heating reflux tank, add 8 liters of 95% ethanol, heat up to 90 ° C and reflux for 2 hours, and cool to temperature At 40°C, release and collect the ethanol extract. Repeat above-mentioned extraction process 3 times, the 95% ethanol amount of the 2nd, 3rd time is respectively 6 liters and 4 liters, and extraction temperature is identical with the 1st time, and extraction time is respectively 1.5 hours and 1 hour, merges 3 times extracting solution, Concentrate under reduced pressure on a rotary evaporator, control the temperature at 45 ° C, concentrate until the specific gravity of the concentrate is 1.3, and obtain 200 grams of extract A;

[0055] 2. Dissolve 200 grams of extract A obtained in step 1 in 500 milliliters of water, then ex...

Embodiment 2

[0059]The method and steps of this embodiment are the same except that step 2 is slightly different from that of embodiment 1. The difference between this example and Example 1 is that the petroleum ether in Step 2 of Example 1 is changed to cyclohexane.

Embodiment 3

[0061] In this example, total flavonoids were separated and extracted from dried eucalyptus flowers, the method and dosage were the same as in Example 1, and about 3.5 grams of total flavonoids were obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a separation and extraction method for separating and extracting total flavone component, goldenrain tree ketone A and goldenratin tree ketone B, they can be used forpreparing medicines for resisting cancers and immune suppression.

Description

technical field [0001] The present invention relates to the separation and extraction method of the total flavonoid parts, two new flavonoid glycosides active ingredients paniculatonoid A (paniculatonoid A) and paniculatonoid B (paniculatonoid B) from the eucalyptus plant, and the invention also relates to their use in anticancer drugs Applications. Background technique [0002] Sapindaceae Koelreuteria Laxm plants, there are 4 species in the world, 3 species in China, 1 variant, and the other in Fiji Islands, deciduous shrubs to trees, wild in forests or cultivated in gardens. Among them, eucalyptus (Koelreuteria paniculata Laxm) is widely distributed in Liaoning from southwest, south China to north China, Taiwan eucalyptus (Koelreuteria henryi Dummer) is exclusively produced in Taiwan Province, compound feather leaf eucalyptus (Koelreuteria bipinnata Franch) is produced in Guangdong, Guangxi, East China and Southwest China The area, commonly known as the money tree. Amon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P37/06A61P39/06C07H1/08C07H17/07
Inventor 林文翰付宏征李军
Owner 林文翰