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Process for preparing thymic peptide

A process method and technology of thymosin, which is applied in the field of improvement of the preparation process, can solve the problems of incompletely broken cells, reduced activity of natural polypeptides, and low yield of thymosin, and achieve the effects of shortening production time, increasing activity, and increasing yield

Inactive Publication Date: 2003-01-15
西安迪赛生物药业有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the above preparation method, the meat grinder and homogenizer are used and repeated freezing and thawing cannot completely break the cells in the thymus. Under neutral conditions, thymosin is easily hydrolyzed by cathepsin, and heating for too long will also destroy thymosin. As a result, not only thymosin The yield is low, and the activity of the natural polypeptide is reduced; in addition, the process takes a long time and the production cost is high

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1kg of fresh calf thymus, add 2000ml of buffer solution with pH 2.5, ultrasonically break at below 10°C for 3 minutes×3 times to check if there are no intact cells, extract thymosin and stir for 15 minutes, heat to 80°C with microwave (5 minutes), Quickly cooled with ice-salt water to below 10°C, refrigerated and centrifuged to obtain 2500ml of centrifuged liquid, which was ultrafiltered with a molecular weight cut-off of 6000, and the filtrate was concentrated to 300ml by nanofiltration, and the content of thymosin was determined to be 10mg / ml. 1 At 3% content, active ERFC increases by 30%.

Embodiment 2

[0033] 1kg of fresh pig thymus, add 3000ml of buffer solution with pH 3.5, crush it with a homogenizer below 10°C, rapidly heat to 80°C for 5 minutes, rapidly cool to below 10°C to extract thymosin and stir for 25 minutes, refrigerate and centrifuge to obtain the centrifugate 3500ml, ultrafiltration with a membrane with a molecular weight cut-off of 10000, and the filtrate was subjected to nanofiltration to obtain 350ml of thymosin raw material, and the measured thymosin content was 16mg / ml, thymosin α 1 The content is 2.95%, and the activity of ERFC increases by 35%.

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Abstract

A process for preparing thymic peptide includes such steps as homogenizing fresh thymus gland, adding buffering solution (pH=2-4) for suppressing enzyme activity, breaking cell under 10 deg.C by ultrasonic waves, homogenizing machine and superfine technique, freeze centrifugalizing, ultrafiltering, nano filtering and concentrating. Its advantages are high output rate, high percentage of natural polypeptide, high activity and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to the improvement technology of the preparation process of thymosin. Background technique [0002] Thymosin is a compound drug containing various peptides extracted from animal thymus. It is an essential factor for the maturation, differentiation and functionalization of T lymphocytes in the animal immune system, and it is an immunomodulator as a drug. The general preparation process of thymosin comprises the following steps: [0003] 1. Homogenize the fresh thymus with a meat grinder and a homogenizer, and freeze and thaw repeatedly to break up the cells; [0004] 2. Extract broken cells with water; [0005] 3. Heat the extract to 85°C and maintain it for 10 minutes, then centrifuge to remove the protein precipitate; [0006] 4. The centrifugate is ultrafiltered through an ultrafiltration column with a cut-off molecular weight of 10,000; [0007] 5. A...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/435C07K14/47
Inventor 郑昌学吴建中徐学平李小学王文
Owner 西安迪赛生物药业有限责任公司
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