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Process for purifying swainsonine by biofermentation

A technology of swainsonine and biological fermentation, applied in the field of bioengineering research, can solve the problems of low swainsonine content and high cost

Inactive Publication Date: 2003-02-12
葛鹏斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically purified swainsonine is mainly derived from locoweed plants. Due to the low content of swainsonine in plants, there is still a gap in the main technical research of purification. If it is artificially synthesized, the cost will be very high

Method used

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  • Process for purifying swainsonine by biofermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: According to the technical scheme of the present invention, the process of purifying swainsonine is carried out in the following steps:

[0022] 1) Preservation of Rhizoctonia legumes 7-3: Inoculate the self-isolated Rhizoctonia legumes 7-3 PDA medium, store in a refrigerator at 4°C, and pass down once every 20 days;

[0023] The formula of PDA medium is: 200g peeled potatoes, 15g agar, 20g glucose, and 1000ml water;

[0024] 2) Rhizoctonia legumes 7-3 inoculation, culture, and mycelium collection: In a sterile state, inoculate 7-3 strains in Czapek's medium for biological fermentation, culture at 25°C for 2 weeks with aeration, and collect hyphae Body, dry, set aside;

[0025] The formula of Czapek’s medium is composed of 30 g sucrose, 3 g sodium nitrate, 1.3 g dipotassium hydrogen phosphate, 0.5 g magnesium sulfate, 0.5 g potassium chloride, 0.01 g ferrous sulfate, 3 g yeast extract, and 1000 ml water.

[0026] 3) Purification of swainsonine from Rhizoctonia le...

Embodiment 2

[0031] Example 2: According to the technical scheme of the present invention, the process of purifying swainsonine is carried out in the following steps:

[0032] 1) Preservation of Rhizoctonia legumes 7-3: Inoculate the self-isolated Rhizoctonia legumes 7-3 in PDA medium, store in a refrigerator at 4°C, and pass down once every 25 days;

[0033] The formula of PDA medium is: 1000g peeled potatoes, 75g agar, 100g glucose, and 5000ml water;

[0034] 2) Rhizoctonia legumes 7-3 inoculation, culture and mycelium collection: In a sterile state, inoculate 7-3 strains in Czapek's medium for biological fermentation, and cultivate for 2 weeks with aeration at 27°C to collect the hyphae Body, dry, set aside;

[0035] The formula of Czapek’s medium is composed of 150 g sucrose, 15 g sodium nitrate, 6.5 g dipotassium hydrogen phosphate, 2.5 g magnesium sulfate, 2.5 g potassium chloride, 0.05 g ferrous sulfate, 15 g yeast extract, and 5000 ml water.

[0036] 3) Purification of swainsonine from...

Embodiment 3

[0041] Example 3: According to the technical scheme of the present invention, the process of purifying swainsonine is carried out according to the following steps:

[0042] 1) Preservation of Rhizoctonia legumes 7-3: Inoculate the self-isolated Rhizoctonia legumes 7-3 PDA medium, store in a refrigerator at 4°C, and passage once every 20 days to 30 days;

[0043] The formula of PDA medium is: 2000g peeled potatoes, 150g agar, 200g glucose, and 10000ml water;

[0044] 2) Rhizoctonia legumes 7-3 inoculation, culture and mycelium collection: In a sterile state, inoculate 7-3 strains in Czapek's medium for biological fermentation, culture at 26°C with aeration for 2 weeks, and collect the hyphae Body, dry, set aside;

[0045]The formula of Czapek’s medium is as follows: sucrose 300g, sodium nitrate 30g, dipotassium hydrogen phosphate 13g, magnesium sulfate 5g, potassium chloride 5g, ferrous sulfate 0.1g, yeast extract 30g, and water 10000ml.

[0046] 3) Purification of swainsonine from...

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Abstract

A biological fermenting process for purifying swainsonine includes such steps as biological fermenting engineering with pulse rhizoctonia 7-3 strain to culture its mycelia containing high content of swainsonine, extracting in alcohol solvent, cationic exchange, silica-gel column chromatography and segmental sublimation. Its advantages are low cost, high purity (more than or equal to 99%) and high output rate.

Description

[0001] 1. Field [0002] The invention belongs to the field of biological engineering research, and specifically relates to biological fermentation, biological pharmacy, natural product extraction, separation and identification methods, and particularly relates to a process for purifying swainsonine by biological fermentation. 2. Background technology [0003] There are three ways for the source of swainsonine: ①Extract and separate it from plants. It is currently known that the plants containing swainsonine are mainly plants of the legume Astragalus and Oxytropis (locoweed), which are widely distributed in All parts of the world, especially North America and my country, are rich in resources; followed by the leguminous swainsonine, which is limited to Australia. ② Extract and separate from Rhizoctonia leguminicola. Schneider et al. (1983) isolated swainsonine from Rhizoctonia legumes for the first time. ③Swainsonine is synthesized artificially. In view of the numerous uses of swai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06
Inventor 童德文曹光荣赵宝玉葛鹏斌
Owner 葛鹏斌
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