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Biosensor

一种生物传感器、过滤器的技术，应用在生物传感器领域，能够解决不能保证恒定、测定不可能、应答值有波动等问题

Inactive Publication Date: 2003-09-24
PANASONIC HEALTHCARE HLDG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the filtered plasma flows into the sensor at a slow rate, it cannot be kept constant, so the response value fluctuates, and when the plasma enters the sensor, air bubbles are often generated, making the measurement impossible

Method used

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Experimental program
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Embodiment 1

[0079] in having Figure 1-4 In the cholesterol sensor of the above structure, the reaction layer 31a contains an electron mediator, the reaction layer 31b contains cholesterol oxidase, cholesterol esterase and a surfactant, and the layer 32 is composed of a surfactant. Make this sensor in the order shown below.

[0080] First, 5 μl of a 0.5 wt % aqueous solution of carboxymethylcellulose sodium salt was dropped on the electrode system of the substrate 1 and dried in a warm air drier at 50° C. for 10 minutes to form the CMC layer 30 . Then, 4 μl potassium ferricyanide aqueous solution (corresponding to potassium ferricyanide 70 mM) was dropped on the CMC layer 30, and dried in a warm air drier at 50° C. for 10 minutes to form a reaction layer 31 a containing potassium ferricyanide.

[0081] Drop 2 μl of a 2 wt% ethanol solution of polyoxyethylene (10) octylphenyl ether (TritonX-100) as a surfactant on the depression formed by the incision of the cover part combining the cover...

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Abstract

The present invention provides an improved biosensor which uses whole blood as the measurement object, and allows blood plasma which has been filtered out of blood cells to reach an electrode system quickly. The biosensor of the present invention has an insulating substrate, an electrode system including a measuring electrode and a counter electrode arranged on the substrate, a reaction layer including at least an oxidoreductase and an electron mediator, a test solution supply channel including the electrode system and the reaction layer, The sample supply part and the filter for filtering blood cells provided between the sample supply part and the test solution supply passage, and the blood plasma that has filtered the blood cells through the above filter can be sucked into the above test solution supply passage by capillary phenomenon . The filter described above is characterized in that the cross-sectional area of ​​the filter is larger than the cross-sectional area of ​​the opening of the above-mentioned test solution supply passage.

Description

technical field [0001] The present invention relates to a biosensor, especially a cholesterol sensor, capable of rapidly, accurately and simply quantifying a specific component in a test solution. Background technique [0002] A glucose sensor will be described as an example of a conventional biosensor. [0003] Generally, the well-known glucose quantification method is a method in which glucose oxidase is combined with an oxygen electrode or a hydrogen peroxide electrode. Glucose oxidase selectively oxidizes substrate β-D-glucose by using oxygen as an electron medium to generate D-glucono-5-lactone, and at the same time, oxygen is reduced to hydrogen peroxide. Glucose can be quantified by measuring the amount of oxygen consumed at this time by an oxygen electrode, or by measuring the amount of hydrogen peroxide produced by using a hydrogen peroxide electrode composed of a platinum electrode or the like. [0004] However, with the above-mentioned method, depending on the o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00G01N33/487G01N33/49G01N33/543
CPCC12Q1/004G01N27/3272
Inventor 长谷川美和山本智浩渡边基一池田信南海史郎
Owner PANASONIC HEALTHCARE HLDG CO LTD