New method for preparing liposome

Abstract

A process for preparing the liposome of medicine includes such steps as dissolving the lipid in tert-butanol, dissolving the medicine in water, proportional mixing, freeze drying, and hydrating. Its advantages are simple process and high stability.

Description

Technical field: [0001] The invention relates to the technical field of biomedicine, in particular it is a new method for preparing liposomes. Background technique: [0002] Liposomes are closed vesicle-like structures formed by phospholipid bilayers. Liposomes can be classified into unilamellar vesicles, multilamellar vesicles and multivesicular liposomes according to their structure. Liposomes were first discovered in 1965 by the British Bangham. Since then, it has been found that liposomes are of great value as drug carriers, so liposomes have been systematically studied. [0003] After more than 20 years of exploration, researchers have proposed many valuable liposome preparation methods. At present, the preparation methods of liposomes can be mainly divided into four categories: 1) Based on the film dispersion method, the phospholipids used to form liposomes are dissolved in organic solvents—usually chloroform or chloroform and methanol mixture, and then remove the ...

AI Technical Summary

Problems solved by technology

For example, the removal of toxic solvents during milking requires special techniques; due to the presence of organic solvents, encapsulating protein or nucleic acid drugs often leads to drug inactivity

3) The detergent dialysis method is difficult for industrial production and is not suitable for encapsulating water-soluble drugs

Formulations intended for intravenous administration may become unusable due to particle size variation

[0006] 2. Due to the characteristics of phospholipids, when phospholipids exist in the water phase, they are prone to hydrolysis and oxidation.

Since phospholipids are hydrolyzed to form lysophospholipids, on the one hand, the toxicity of the preparation is increased, and on the other hand, liposomes are easily disintegrated, resulting in leakage of the encapsulated drug.

[0007] 3. If the liposome is suspended in the water phase, the drug will leak slowly during storage, resulting in a change in the encapsulation rate of the drug

This often results in complex production processes

Moreover, the heat generated by these means can also cause oxidation and hydrolysis of phospholipids