New method for preparing liposome

A liposome and new method technology, applied in the field of biomedicine, can solve the problems of loss of drug activity, increased toxicity of preparations, complicated production process, etc.

Inactive Publication Date: 2003-10-01
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the removal of toxic solvents during milking requires special techniques; due to the presence of organic solvents, encapsulating protein or nucleic acid drugs often leads to drug inactivity
3) The detergent dialysis method is difficult for industrial production and is not suitable for encapsulating water-soluble drugs
Formulations intended for intravenous administration may become unusable due to particle size variation
[0006] 2. Due to the characteristics of phospholipids, when phospholipids exist in the water phase, they are prone to hydrol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of tert-butanol / water / lipid three-phase diagram. Dissolve fat in preheated tert-butanol to obtain fat / tert-butanol solutions with different concentrations, dilute the solutions with different concentrations with different amounts of water, place them at constant temperature, record the phase boundary point, and make a phase diagram. It is found that at 10 degrees, 20 degrees, and 35 degrees, the concentration of phospholipid / tert-butanol solution is less than 250 mg / ml, and the phase boundary point is basically at about 3 / 7 of tert-butanol / water.

Embodiment 2

[0048] The influence of the carrier substance sucrose on the characteristics of the freeze-dried product: the lipid / tert-butanol solution and the sucrose / water solution were mixed to obtain a single-phase solution for freeze-drying. It was found that the addition of sucrose significantly improved the shape of the freeze-dried product and accelerated the hydration. And the obtained freeze-dried product can be stored for a long time without absorbing moisture.

Embodiment 3

[0050] Effect of carrier substance sucrose on liposome particle size: Lipid / tert-butanol solution and sucrose / water solution were mixed to obtain a monophasic solution and lyophilized. The mass ratios of phospholipids / sucrose in the freeze-dried product were 1:1, 1:2.5, 1:5, 1:7.5, respectively. Liposomes with a phospholipid concentration of 1% were formed after adding water. The particle size was measured with ZETASIZER (Malvern). The particle diameters of the liposomes were 304.3nm, 146.0nm, 131.6nm, and 129.8nm (intensity mean). This shows that adding carrier substance sucrose can change the particle size of liposome preparation solution.

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Abstract

A process for preparing the liposome of medicine includes such steps as dissolving the lipid in tert-butanol, dissolving the medicine in water, proportional mixing, freeze drying, and hydrating. Its advantages are simple process and high stability.

Description

Technical field: [0001] The invention relates to the technical field of biomedicine, in particular it is a new method for preparing liposomes. Background technique: [0002] Liposomes are closed vesicle-like structures formed by phospholipid bilayers. Liposomes can be classified into unilamellar vesicles, multilamellar vesicles and multivesicular liposomes according to their structure. Liposomes were first discovered in 1965 by the British Bangham. Since then, it has been found that liposomes are of great value as drug carriers, so liposomes have been systematically studied. [0003] After more than 20 years of exploration, researchers have proposed many valuable liposome preparation methods. At present, the preparation methods of liposomes can be mainly divided into four categories: 1) Based on the film dispersion method, the phospholipids used to form liposomes are dissolved in organic solvents—usually chloroform or chloroform and methanol mixture, and then remove the ...

Claims

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Application Information

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IPC IPC(8): A61K9/127
Inventor 李春雷邓英杰
Owner SHENYANG PHARMA UNIVERSITY
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